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Heavy-metal-resistant rhizobium and application thereof

A rhizobia and heavy metal technology, applied in the fields of agriculture and environmental pollution control, can solve the problems of extreme lack of nutrient elements, poor soil, and high residual heavy metal concentration, so as to improve the nodulation rate and nitrogen content, improve stress resistance, promote effect of plant growth

Inactive Publication Date: 2015-04-08
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, due to many limiting factors in abandoned mines, such as poor soil, high concentration of residual heavy metals, extreme acidity, extreme lack of macronutrients, etc., the vast majority of abandoned mines lack natural vegetation growth.

Method used

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  • Heavy-metal-resistant rhizobium and application thereof
  • Heavy-metal-resistant rhizobium and application thereof
  • Heavy-metal-resistant rhizobium and application thereof

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Embodiment Construction

[0023] It should be noted that the rhizobium HZ76 involved in the present invention is classified as Mesorhizobium sp.HZ76, and the strain of this rhizobium was preserved in the China Center for Type Culture Collection, referred to as CCTCC, and registered on December 26, 2014. The biological strain was preserved for 30 years from December 26, 2014. The preservation number of Rhizobium HZ76 is: CCTCC NO: M 2014667.

[0024] The isolation and identification method of rhizobia of the present invention is as follows:

[0025] Rhizobium HZ76 is an endophytic bacterium isolated from a root nodule of Robinia pseudoacacia growing in a lead-zinc smelter in Shaanxi Province, China, and identified as Mesorhizobium sp. The main biological characteristics are: G-, the thallus is rod-shaped when young, without spores. It grows well on YMA medium, and the single colony is round, with neat edges, 1-2 mm in diameter, and transparent.

[0026] Select a large and plump nodule, cut it off wit...

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Abstract

The invention discloses a heavy-metal-resistant rhizobium namely rhizobium HZ76. The rhizobium is applied to promoting plants to repair heavy metal polluted soil. The effective bacterium number of rhizobium HZ76 strains is (10<8>-10<9>) CFU / mL. A plant adopted by plant repair of the heavy metal polluted soil is locust tree. The heavy metals in the heavy metal polluted soil comprise one or more of Cu, Zn, Pb and Cd. The HZ76 strain disclosed by the invention can produce indoleacetic acid which is beneficial to promoting plant growth. The heavy-metal-resistant rhizobium has an ACC deaminase gene and nod and nif genes, which are helpful for improving the nodulation rate and nitrogen content of plants. The heavy-metal-resistant rhizobium can generate an iron carrier which is helpful for improving the stress resistance of the plants. Plant growth promoting bacteria is used for promoting the growth of the plants, improving the content of the heavy metals Zn, Pb and Cu in the plants, and strengthening the plant extraction repair efficiency.

Description

technical field [0001] The invention belongs to the fields of agriculture and environmental pollution control, and relates to rhizobia, in particular to a heavy metal-resistant rhizobia and application thereof. Background technique [0002] Heavy metals remain in the soil for a long time and have great harmful effects. They are easily absorbed by crops and enter the food chain to endanger human health. Therefore, with the intensification of soil heavy metal pollution on a global scale, how to economically and efficiently control and control soil pollution has become the focus of many related fields at home and abroad. Phytoremediation has become an important means of remediating heavy metal-contaminated environments in recent years due to its low cost, simplicity, safety, reliability, and environmental friendliness. However, due to many limiting factors in abandoned mines, such as poor soil, high concentration of residual heavy metals, extreme acidity, and extreme lack of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00B09C1/00C12R1/01
CPCA01N63/00B09C1/105C12N1/205C12R2001/01
Inventor 林雁冰樊妙春赵亮韦革宏
Owner NORTHWEST A & F UNIV
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