Separation medium for purifying proteins, and preparation method thereof

A separation medium and protein technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as fragility, poor strength, and large pressure drop in column operation, and achieve large adsorption capacity and large specific surface area , the effect of high adsorption rate

Inactive Publication Date: 2015-04-08
CHENGDU JINHUI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned separation medium has the problems of soft matrix, poor strength, easy breakage during use, large pressure drop in column operation, poor separation specificity for protein, low purity of the obtained purified product, and poor product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A separation medium for purifying proteins, comprising the following raw materials in parts by weight:

[0038] Oil phase:

[0039] Glycidyl methacrylate 40 parts

[0040] 15 parts of ethylene glycol dimethacrylate

[0041] Triallyl isocyanurate 5 parts

[0042] 3 parts divinylbenzene

[0043] 5 parts of hydroxypropyl acrylate

[0044] 50 parts of toluene

[0045] gasoline 10 parts

[0046] 10 parts isoamyl alcohol

[0047] Azobisisobutyronitrile 3 parts

[0048] water box:

[0049] 500 parts of deionized water

[0050] 5 parts polyvinyl alcohol

[0051] 3 parts gelatin

[0052] Sodium chloride 50 parts

[0053] 3 parts methylene blue.

Embodiment 2

[0055] A separation medium for purifying proteins, comprising the following raw materials in parts by weight:

[0056] Oil phase:

[0057] Glycidyl methacrylate 50 parts

[0058] 20 parts of ethylene glycol dimethacrylate

[0059] Triallyl isocyanurate 10 parts

[0060] 5 parts divinylbenzene

[0061] 10 parts of hydroxypropyl acrylate

[0062] 60 parts of toluene

[0063] gasoline 20 parts

[0064] 20 parts of isoamyl alcohol

[0065] Azobisisobutyronitrile 5 parts

[0066] water box:

[0067] 1000 parts of deionized water

[0068] 10 parts polyvinyl alcohol

[0069] 5 parts gelatin

[0070] Sodium chloride 100 parts

[0071] 5 parts methylene blue.

Embodiment 3

[0073] A separation medium for purifying proteins, comprising the following raw materials in parts by weight:

[0074] Oil phase:

[0075] Glycidyl methacrylate 45 parts

[0076] Ethylene glycol dimethacrylate 13 parts

[0077] Triallyl isocyanurate 7.5 parts

[0078] 4 parts divinylbenzene

[0079] 7.5 parts of hydroxypropyl acrylate

[0080] 55 parts of toluene

[0081] gasoline 15 parts

[0082] 15 parts isoamyl alcohol

[0083] Azobisisobutyronitrile 4 parts

[0084] water box:

[0085] 750 parts of deionized water

[0086] 7.5 parts of polyvinyl alcohol

[0087] 4 parts gelatin

[0088] Sodium chloride 75 parts

[0089] 4 parts methylene blue.

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Abstract

The invention relates to a separation medium for purifying proteins, and a preparation method thereof, and belongs to the technical field of polymer materials. The separation medium for purifying proteins includes an oil phase and a water phase, wherein the oil phase comprises glycidyl methacrylate, ethylene dimethacrylate, triallyl isocyanurate, divinyl benzene, hydroxypropyl acrylate, toluene, gasoline, isoamyl alcohol and azoisobutyronitrile; and the water phase comprises deionized water, polyvinyl alcohol, gelatin, sodium chloride and methylene blue. The separation medium is prepared by selecting suitable monomers through a polymerization reaction, has the respective advantages of natural saccharide and synthetic polymer separation media, also has the advantages of macro-porous structure, high adsorption rate, large specific surface area and large adsorption capacity, and is separation medium having excellent performances and used for separating and purifying the proteins. The adsorption capacity of the medium provided by the invention to bovine serum albumin (BSA) can reach 171.20mg/g dry resin.

Description

technical field [0001] The invention relates to a separation medium and a preparation method thereof. More specifically, the invention relates to a separation medium for purifying proteins and a preparation method thereof, belonging to the technical field of polymer materials. Background technique [0002] With the increasing demand for biological products, the performance requirements for the separation and purification media of biological products are also getting higher and higher. Natural sugars and polymer synthetic separation media machines have their own advantages and disadvantages. Therefore, it has become a very important research work to prepare a separation and purification medium with the advantages of both. [0003] On July 23, 2008, the State Intellectual Property Office disclosed an invention patent with the publication number CN101224414 and the name "multipurpose separation medium with tetrazole as functional group and its preparation method". Multipurpos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/32C08F222/14C08F226/06C08F212/36C08F220/28C07K1/22C07K1/18
Inventor 张一帆
Owner CHENGDU JINHUI TECH
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