Detection reagent, preparation and detection method for epithelial tissue tumor cells

Abstract

The invention provides a detection reagent for epithelial tissue cancer cells and a preparing and detecting method. The detection reagent for the epithelial tissue cancer cells is high in detecting sensitivity and reliable in detecting result. According to the detecting method, a whole-process sealing pushing-injecting mode is used, stimulation on a patient from special stink from a solution is avoided, meanwhile, time of solution and air contacting is shortened, a false positive result caused by quick oxidation is avoided, and detecting result accuracy is fully guaranteed. The detection reagent for the epithelial tissue cancer cells comprises, by weight, the components of 0.03%-2% of methylene blue, 5%-10% of fructose, 0.1%-1% of ascorbic acid, 1%-5% of folic acid, 40% of dimethyl sulfoxide, 3%-5% of acetic acid-sodium acetate buffer solution, 0.1%-1% of surface active agent and the balance of purified water.

Description

technical field [0001] The invention relates to a detection reagent, in particular to a detection reagent for epithelial tissue tumor cells, and also relates to a preparation method and a detection method of the detection reagent. Background technique [0002] At present, the methods for cell-specific staining of epithelial tissues mainly include acetic acid albinism reaction, iodine test, and methylene blue staining. Special staining liquid has a long history of external diagnosis of diseases. It is an invasive in vitro staining diagnosis method and is widely used in the primary screening of routine lesions such as epithelial tissue. [0003] Acetic acid albinism is applied on the epithelial tissue by 3%-5% acetic acid solution, wait for a period of time to observe whether there is any albinism in the smeared epithelial tissue, if there are tumor cells, this method has poor specificity and is easy to cause dildo. The iodine test is based on the glycogen response and is al...

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Problems solved by technology

[0004] Patent CN103808551A uses the siphon method to store the solution in the cotton swab rod, the solution is easy to seep and oxidize during transportation, and the dyeing solution flows out slowly during use, which is inconvenient to operate

[0005] Chinese patent: 2014100628449 mentions that acetic acid is used to adjust the pH of the dyeing solution. Our research found that when the amount of acetic acid reaches 3%-5%, it is easy to be oxidized, and the pH value also drops to about 3, which does not meet the dyeing requirements. Solution pH5.0-6.0, if the acetic acid content is less than 3%-5%, the acetic acid whitening reaction cannot be realized, and the dual positioning of cotton swab staining and acetic acid whitening reaction of tumor tissue cannot be realized

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