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Molecular specificity labeled primer for identifying T. chinensis seedling and method

A technology of Taxus chinensis and marker primers, which is applied in the field of rapid identification of Taxus chinensis, can solve the problems of difficulty in accurate identification and the like, and achieves the effects of high sensitivity and simple method.

Active Publication Date: 2015-04-01
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Therefore, it is difficult to accurately identify the three similar species by PCR amplification with general ITS primers (ITS4 / ITS5) and ordinary electrophoresis

Method used

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  • Molecular specificity labeled primer for identifying T. chinensis seedling and method

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Embodiment 1

[0027] 1. Extraction of the genomic DNA of the yew sample to be tested

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Abstract

The invention relates to a molecular specificity labeled primer for identifying a T. chinensis seedling and a method. An upstream primer NHSF of the molecular specificity labeled primer for identifying T. chinensis and two similar species including T. madia and T. cuspidata is represented as SEQ ID NO.1, and downstream NHSR is represented as SEQ ID NO.2; and by means of the molecular specificity labeled primer MHSF / MHSR, the T. chinensis and the two similar species of the T. chinensis can be rapidly subjected to molecular identification with a conventional PCR (polymerase chain reaction) method. The method is simple, the PCR technology is adopted for detection, time is short, and the detection can be finished by half day; and the molecular specificity labeled primer and the method provide effective assistance for identification of the T. chinensis and the two similar species of the T. chinensis according to morphological characteristics.

Description

technical field [0001] The invention relates to a method for identifying southern yew Taxus chinensis Molecular-specific marker primers and a method for rapid identification of Taxus chinensis by using the specific primers. Background technique [0002] Southern yew Taxus chinensis It is the gymnosperm subphylum (Cymnospermai), Coniferopside (Coniferopside), Taxales (Taxales), Taxaceae (Taxaceae) Taxus genus ( Taxus ) plants are unique Quaternary relic plants in my country, mainly distributed in the Yangtze River Basin, Nanling Mountains, and mountains or valleys in Henan, Shaanxi, Qinling, Gansu and other provinces. Southern yew has both important ornamental value and important medicinal value. Paclitaxel contained in it is an important drug for treating cancer. Due to the low natural reproduction and regeneration capacity and excessive deforestation by humans, the number of southern yews has become less and less, and it is currently in an endangered state. During the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 沈晨佳冯尚国王慧中
Owner HANGZHOU NORMAL UNIVERSITY
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