Method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes

Abstract

The invention relates to a method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes. The method comprises the following steps: designing primers capable of covering full lengths of GyrA, lnhA, katG and rpoB genes; merging the four gene primers and forming a primer pool; amplifying amplicons capable of covering full lengths of drug-resistance-related genes; establishing complete an SV-MTB library; and detecting and acquiring drug-resistant sites. By the method, multiple mutation sites of a multiple-drug-resistant strain can be detected at one time and tuberculosis patients can be scientifically, safely and accurately guided to take the medicine.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and more specifically relates to a detection method for four tuberculosis drug-resistant genes of rifampicin, isoniazid and fluoroquinolones. Background technique [0002] At present, in the diagnosis and treatment of tuberculosis, tuberculosis drug resistance test is still an important technology, but there are still some problems in the traditional detection method of tuberculosis drug resistance related genes: [0003] 1. The traditional detection method of tuberculosis resistance-related genes can only detect a small number of specific sites at the same time, and cannot determine the mutation nature. [0004] 2. Many drug-resistant strains in actual clinical samples are drug-resistant, and there may be several strains resistant to different drugs in the strain group. Moreover, due to the uneven distribution of bacterial strains, the traditional drug-resistant site detection method can det...

AI Technical Summary

Problems solved by technology

[0003] 1. The traditional detection method of tuberculosis drug resistance-related genes can only detect a small number of specific sites at the same time, and cannot determine the mutation nature

[0004] 2. Many drug-resistant strains in actual clinical samples are drug-resistant, and there may be several strains resistant to different drugs in the strain group

Moreover, due to the uneven distribution of bacterial strains, the traditional drug-resistant site detection method can detect mutation sites with high mutation signals, that is, the dominant group in the bacterial strain. There will be an obvious improvement in the condition, but with the decline of the dominant strains, the population of the disadvantaged strains will slowly transform into the dominant strains, and there will be significant deterioration of the condition in the later stage of treatment

Cannot scientifically, safely and accurately guide the medication of tuberculosis patients

Images