Method for breeding drought-resistant hybrid rice
A hybrid rice and rice seed technology, applied in the field of drought-resistant hybrid rice breeding, can solve problems such as food security crisis, semi-aquatic plant stress, and difficulty in drought-resistant breeding, and achieve the goal of expanding genetic distance, protecting the ecological environment, and saving water resource effect
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Embodiment 1
[0020] A breeding method for drought-resistant hybrid rice. The C4 plant barnyardgrass with strong adaptability, humidity-loving, warm-loving, and drought-tolerant in Chongqing is selected as the donor, and the comprehensive agronomic technique developed by the "Yuyou" rice team of Chongqing Academy of Agricultural Sciences is used. The restorer lines Yuhui 7109R, 7108 R and the maintainer line 805B with excellent traits were the recipient parents;
[0021] (1) Introduction of barnyardgrass exogenous DNA: in the first spring in Chongqing, the total DNA of barnyardgrass was introduced into Yuhui 7109, Yuhui 7108, and maintainer line Yu 805B by using the pollen tube introduction method;
[0022] (2) Observation and selection of barnyardgrass DNA-introduced progeny materials: Plant the imported progeny materials in Hainan in the autumn of the first year and harvest the seeds per plant (D1); in the spring of the second year, plant each individual plant in a single-plant row area in...
Embodiment 2
[0029] The restorer lines 21281 (R7108×Barnyardgrass), 21288 (R7108×Barnyardgrass), 10486 (R7109×Barnyardgrass) bred in Hainan in the autumn of 2013 and Yu 650A, Yu 802A, Yu 805A Wait for CMS testing and mating.
[0030] In the spring of 2014, it was planted in the Yuxi Science and Technology Demonstration Park of Chongqing Academy of Agricultural Sciences, Yongchuan District, Chongqing: it was sown on March 6, the seedlings were raised with plastic film, and the seedlings were transplanted on April 17. The planting size was 33cm×20cm, and each combination Plant 5-10 rows, 12 litters per row. Irrigation of shallow water in the field within 10 days after transplanting is conducive to the rejuvenation and survival of the seedlings. After the seedlings are alive and drained until the rice matures, the field will no longer be artificially irrigated; before the rice is harvested, 5 litters with the average number of spikes are used to investigate the traits of spikes and grains. 6...
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