Single-frame light intensity transmission quantitative phase microscope system and method

Abstract

The invention discloses a single-frame light intensity transmission quantitative phase microscope system and method. The light splitting is realized by utilizing a Michelson structure, digital defocus can be carried out by adopting a space light modulator, the mechanical movement caused by adopting a defocus strength image in a traditional method can be avoided, the acquired series of light intensity is distributed, and the quantitative acquisition of the phase information can be realized by solving the light intensity transmission equation. By adopting the system and the method, the mechanical movement caused by acquiring the defocus strength image in the traditional method can be avoided, the acquisition speed and accuracy of the system can be greatly improved, the application range of the light intensity transmission equation method can be effectively enlarged from a static slowly-varied object to a high-speed dynamic object, and the high-speed and high-resolution three-dimensional quantitative phase microscope imaging can be realized.

Description

technical field [0001] The invention belongs to optical measurement and imaging technology, in particular to a dynamic quantitative microscopic imaging device and method based on light intensity transmission equation. Background technique [0002] Phase recovery is an important topic in optical measurement and imaging technology, and phase imaging technology is playing an important role in both biomedical and industrial detection fields. Throughout the progress of optical measurement for nearly half a century, the most classic phase measurement method should be none other than interferometry. However, the disadvantages of interferometry are also very obvious: interferometry generally requires a highly coherent light source (such as a laser), which requires a more complex interferometric device; the introduction of an additional reference optical path leads to very harsh requirements for the measurement environment; The speckle coherent noise introduced by highly coherent li...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a single-frame light intensity transmission quantitative phase microscopy system and method thereof, to solve the quantitative phase microscopic imaging problem based on the light intensity transmission equation, and effectively change the application range of the light intensity transmission equation from static to static Slowly changing objects are extended to high-speed dynamic objects, realizing high-speed and high-resolution three-dimensional quantitative phase microscopy imaging

Images