Structure and application of three-dimensional fluorescence standard spectrum library used for recognizing toxic-to-fish algae

Abstract

The invention discloses a structure and an application of a three-dimensional fluorescence standard spectrum library used for recognizing toxic-to-fish algae. The hemolytic activity and the change of the toxic-to-fish algae in different growing periods and under the control of environmental factors, and a chlorophyll three-dimensional fluorescence spectrum of the toxic-to-fish algae are researched and contrasted with a chlorophyll three-dimensional fluorescence spectrum of large sample nontoxic-to-fish algae, three-dimensional fluorescence spectrum analysis and recognition methods are screened, a fluorescence characteristic spectrum closely related to the toxic-to-fish algae and the hemolytic activity of the toxic-to-fish algae is extracted, and three-dimensional fluorescence standard spectrum libraries of the toxic-to-fish algae and the nontoxic-to-fish algae are screened by a clustering method; based on the three-dimensional fluorescence standard spectrum libraries, a Fisher discrimination function for recognizing the toxic-to-fish algae and a function for discriminating the degree of the hemolytic activity of the toxic-to-fish algae are established respectively. Discrimination results obtained by utilizing the discrimination functions are more stable, accuracy and reliable. The method provided by the invention realizes highly correct diagnosis and recognition functions for the toxic-to-fish algae in an in-place red tide water body and the hemolytic activity of the toxic-to-fish algae.

Description

technical field [0001] The invention relates to the construction of a standard spectral library, in particular to a construction method and application of a three-dimensional fluorescent standard spectral library for identifying fish-toxic algae. Background technique [0002] With the rapid development of my country's coastal economy and society, the ocean transportation capacity has been continuously enhanced, the eutrophication of coastal waters and alien biological species have increased sharply, and the toxic and harmful red tides are on the rise, which has given my country's sustainable and stable development of coastal aquaculture industry and Seafood food safety poses a huge threat. In recent years, fish-toxic algae red tides have occurred almost every year along the coast of my country, causing huge economic losses to marine aquaculture. A large number of abalones and large yellow croakers died, and the economic loss was as high as more than 2 billion yuan. In October...

AI Technical Summary

Problems solved by technology

This method pioneered the application of three-dimensional fluorescence technology to the identification of fish-toxic algae, but because the database of the established model is only three kinds of fish-toxic algae, and the nutritional factor only selects iron ions, the established three-dimensional fluorescence spectrum database has limited data. The method is only a preliminary research result, and the correct discrimination rate of fish-toxic algae in the water body of the red tide site is not high (about 65% on average)

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