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Method for hatching diapause brine shrimp eggs under blue light

A technology for Artemia eggs and diapause, which is applied in the field of hatching Artemia diapause eggs under blue light, can solve the problem of low hatching rate of Artemia eggs under weak light, and achieve the effect of improving the hatching rate and solving the low hatching rate

Inactive Publication Date: 2015-01-21
TIANJIN OCEAN PAL CAROL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The problem to be solved by the present invention is to provide a method for hatching diapause Artemia eggs under blue light. This method not only realizes the hatching problem of diapause Artemia eggs under blue light environment, but also solves the hatching rate of Artemia eggs under weak light. Low problem, and at the same time ensure the stability of hatching rate, the present invention is also suitable for ensuring stable hatching rate in large hatching places

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 22g dissolved salt / L, pH is 8.5, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 28°C;

[0020] The liquid surface of the hatching solution is irradiated with blue light, and the light intensity of the blue light is 1800Lux.

[0021] The density of the diapause Artemia eggs in the hatching solution is 2.7 mg / L; and the Artemia eggs of Kazakhstan are used.

[0022] After 24 hours of incubation, the hatching rate of Artemia eggs reached 89%.

Embodiment 2

[0024] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 22g dissolved salt / L, pH is 8.5, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 28°C;

[0025] The liquid surface of the hatching solution is irradiated with blue light, and the light intensity of the blue light is 1800Lux.

[0026] The density of the diapause Artemia eggs in the hatching solution is 2.7 mg / L; and the shelled Artemia eggs of Kazakhstan are used.

[0027] After 24 hours of incubation, the hatching rate of Artemia eggs reached 92%.

Embodiment 3

[0029] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water. The salinity is 22g dissolved salt / L, the pH is 8.5, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 28°C; 180mg / L is added to the hatching solution of tea polyphenols.

[0030] The liquid surface of the hatching solution is irradiated with blue light, and the light intensity of the blue light is 1800Lux.

[0031] The density of the diapause Artemia eggs in the hatching solution is 2.7 mg / L; and the shelled Artemia eggs of Kazakhstan are used.

[0032] After 24 hours of incubation, the hatching rate of Artemia eggs reached 94%.

[0033] The Kazakhstan artemia ovum of shelling treatment used in embodiment two and embodiment three all obtains through following processing steps:

[0034] 1) Configuration ...

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PUM

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Abstract

The invention provides a method for hatching diapause brine shrimp eggs under blue light and solves the problem that the hatching rate of the brine shrimp eggs under weak light is low. The method has the advantages that diapause brine shrimp egg hatching under the blue light is achieved, diapause brine shrimp egg hatching rate under the blue light can be increased by controlling factors such as light intensity, temperature, pH value and dissolved oxygen, the hatching rate in 24 hours can reach more than 89%, stable hatching rate is guaranteed, and stable hatching rate of large hatcheries is also guaranteed.

Description

technical field [0001] The invention relates to the technical field of Artemia hatching, in particular to a method for hatching diapause eggs of Artemia under blue light. Background technique [0002] Artemia nauplii is generally used as live feed for aquaculture, especially as live feed for marine fish and sea shrimp larvae in the early stage. Generally, there is no such live feed for Artemia nauplii in the market, only Artemia nauplii eggs, nauplii It is hatched from Artemia eggs. The hatching of Artemia eggs can be completed in the hatching solution, but at present, people have different opinions on the salinity, pH, light intensity, and incubation time required for Artemia hatching. Most of the hatching research focuses on the above aspects. No one has yet proposed the effect of different energy lights on the hatching of Artemia. By studying the hatching of Artemia eggs under blue light, the hatching of Artemia eggs under low light can be achieved, and stability can be g...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 孙朦朦柴慈民王永苓
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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