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Method applied to tissue culture and rapid propagation of chimonanthus nitens

A technology for tissue culture and wintersweet, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of easy browning, difficult rooting, vitrification and other problems of wintersweet tissue culture, and achieve low browning rate, High survival rate and fast rooting effect

Active Publication Date: 2015-01-21
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, there are problems such as easy browning, vitrification and rooting difficulties in wintersweet tissue culture.

Method used

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  • Method applied to tissue culture and rapid propagation of chimonanthus nitens
  • Method applied to tissue culture and rapid propagation of chimonanthus nitens
  • Method applied to tissue culture and rapid propagation of chimonanthus nitens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for tissue culture and rapid propagation of Myrtle japonicus, comprising the following steps in turn:

[0022] (1) Obtaining sterile vaccines:

[0023] Take the mature waxberry fruit, peel off the oblong achene, cut off a little at each end of the achene with surgical scissors, be careful not to cut the seed coat or damage the seed, and carefully peel off the achene with the complete seed coat with your fingernails. Seeds, soaked in 0.2% HgCl in a clean bench 2 Disinfect in the solution for 17-40 minutes (too short time is easy to contaminate, too long time is easy to cause seed death), take it out, soak in sterile water and rinse 3 times, each time for 5 minutes, inoculate in Nitsch+GA 3 0.1 mg / L +6-BA 2.0 mg / L+NAA 0.2mg / L (“+” means mixed). The added amount of sucrose in this medium was 30 g / L, the added amount of agar was 7 g / L, and the pH was 5.8-6.0. The medium was sterilized at 121°C for 20 minutes by high temperature and high pressure, and cultured in...

Embodiment 2

[0044] Effects of Different Medium Additives on Callus Induction and Adventitious Bud Induction

[0045] The difference between this example and example 1 lies in step (2): cut off the cotyledons and divide them into 1 cm sections with a scalpel. 2 Small pieces of about the size are inoculated in the medium of Nitsch+TDZ 0.1-2.0 mg / L+NAA 0-2.0 mg / L+PVP 0-5.0 mg / L+LH 0-1.0 mg / L, cultured in light culture Indoors, the culture temperature was (25±1)°C, and after every 14 hours of light culture, it was transferred to the dark for 10 hours, and the light intensity was 30-40 μmol / (m 2 s). The cotyledons began to expand at 8-15 days, yellow-green dense callus began to appear at 11-25 days, and adventitious buds differentiated from the callus at 28-40 days, the callus induction rate was 70-100%, and the differentiation rate was 28 -70%, some explants browned after the callus was produced, and the browning rate was 5-45%. Nitsch+TDZ 0.5 mg / L+NAA 0.2 mg / L+PVP 1.0 mg / L+LH 0.5 mg / L was...

Embodiment 3

[0050] Effects of Different Proliferation Medium Additives on Proliferation of Adventitious Buds and Strong Seedlings

[0051] The difference between this example and Example 1 lies in step (3): Cut the differentiated shoots from the callus and transfer to the proliferation medium Nitsch+TDZ 0-2.0 mg / L+6-BA 0-5.0 mg / L+CH 0-5.0 mg / L+PVP 1.0 mg / L for proliferation culture, the multiplication factor can reach 3.5-7.2 in 25 days. Among them, Nitsch+TDZ 0.3 mg / L +6-BA 1.0 mg / L+CH 2.0 mg / L+PVP 1.0 mg / L had the best effect, the multiplication factor was 7.0, no vitrification occurred, and the buds grew normally and the leaf color normal. Although the addition of CH did not significantly increase the proliferation rate, it slightly promoted the proliferation rate, and also promoted the adventitious buds' strong seedlings. Generally speaking, the reason for vitrification is that the concentration of the hormone used is too high. As long as the hormone is adjusted at an appropr...

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Abstract

The invention discloses a method applied to tissue culture and rapid propagation of chimonanthus nitens. The method sequentially comprises the following steps: obtaining aseptic seedlings; taking mature chimonanthus nitens fruits and peeling seeds with complete seed coat; inducing callus and inducing adventitious buds; cutting off cotyledon, cutting the cotyledon into small slices with area of 1 square centimeter by a scalpel, inoculating the small slices in a minimal medium; proliferating and seedling the adventitious buds; cutting off the differentiated buds from the callus, and transferring the differentiated buds into a proliferation culture medium to carry out proliferation culture; rooting rootless seedlings; cutting off strong adventitious buds when the adventitious buds grow to be 3-4cm high, and inoculating the strong adventitious buds in a rooting culture medium; hardening and transplanting seedlings of tissue culture regeneration plants; hardening the seedlings when the seedlings of to-be-rooted tissue culture regeneration plants are 5cm high, the root number is greater than 3 and the root length is greater than 5cm, pounding the culture medium and taking out the plants after three days and burying the roots of the regeneration plants in the culture soil. The chimonanthus nitens cultured by the method is capable of quickly rooting and is low in vitrifying and browning rate and high in survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a rapid propagation method of wintersweet. Background technique [0002] Wintersweet ( Chimonanthus nitens Oliv.), also known as stinky wintersweet, autumn wintersweet, bright-leaf wintersweet, wild wintersweet, etc., is an evergreen shrub of the family Cesaceae, produced in Anhui, Zhejiang, Jiangsu, Jiangxi, Fujian, Hubei, Hunan , Guangxi, Yunnan, Guizhou and Shaanxi provinces. The leaves are used as medicine, famous winter plum, different names Xiangfeng tea, Mao camellia, rock horse mulberry, it is warm in nature, pungent, slightly bitter, has the effect of dispelling wind and relieving exterior, aromatizing dampness, and mainly treating influenza, heat stroke, chronic bronchitis , Dampness, chest tightness and mosquito bites embolism. Wintersweet leaves have a strong fragrance, and its essential oil components not only have analgesic, antitus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱诚孙骏威王飞娟江琼丁艳菲
Owner CHINA JILIANG UNIV
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