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Application of pik3c2a protein in the treatment of liver cancer

A technology for the treatment of liver cancer and drugs, which can be used in drug combinations, gene therapy, peptide/protein components, etc., and can solve problems such as invasion and metastasis of liver cancer cells

Active Publication Date: 2017-06-30
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to provide an application of PIK3C2A protein in the treatment of liver cancer drugs to solve the technical problem of postoperative liver cancer cell invasion and metastasis in liver cancer patients in the prior art

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  • Application of pik3c2a protein in the treatment of liver cancer
  • Application of pik3c2a protein in the treatment of liver cancer
  • Application of pik3c2a protein in the treatment of liver cancer

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Embodiment 1

[0117] animal test

[0118] experimental method:

[0119] 1. Subcutaneous tumor formation experiment in nude mice

[0120] BALB / C-nu / nu immunodeficient mice were purchased from Changsha Jingda Experimental Animal Company and fed in an SPF-grade environment in the Department of Zoology, Central South University. Referring to the experimental method of Xia L et al., the following operations were carried out: the cells in good condition were digested with trypsin and then made into a suspension with saline, and the concentration of the cells was adjusted to 3×107 to 6×107 / ml. 200 μl of cell suspension was extracted and injected into the axilla of the right forelimb. From the next day, the difference in the growth rate of subcutaneous tumors between the two groups was compared by measuring the long and short diameters of the subcutaneous tumors every day. One month later, when the subcutaneous tumor grew to a certain size, the nude mice were killed by cervical dislocation, the su...

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Abstract

The invention provides an application of a PIK3C2A protein in a medicament for treating liver cancer. According to the application provided by the invention, expression levels of PIK3C2A mRNA and proteins in liver cancer tissues and liver cancer cell lines are determined by virtue of a real-time PCR and protein immunoblotting technique firstly; then a difference of PIK3C2A staining degrees in the liver cancer tissues and adjacent nontumorous liver tissues (adjacent nontumorous liver tissue, ANLT) are compared by applying an immunohistochemical method; and combined with clinical follow-up data, statistical methods including Kaplan-Meier survival analysis, Log-Rank test, Spearman correlation analysis, and single / multi-factor correlate analysis of COX regression are adopted to define relationships between the expression level of PIK3C2A as well as pathological features and poor prognosis of the liver cancer. With the adoption of the application, the expression of the PIK3C2A is lowered by adopting a lentivirus transfection technique, and regulating function of the PIK3C2A in growth and migration movement of the liver cancer cells is verified by virtue of a series of function experiments in vivo and vitro.

Description

technical field [0001] The present invention relates to the field of liver cancer treatment, in particular, to the application of a PIK3C2A protein in medicine for treating liver cancer. Background technique [0002] Hepatocellular carcinoma ranks second among malignant tumors in men and sixth among malignant tumors in women worldwide, and is the third leading cause of death from malignant tumors in the world. The annual death cases in China account for about 100% of the total number of deaths due to liver cancer in the world 55%, so liver cancer has become a major obstacle limiting the further improvement of Chinese people's health. With the development of surgical techniques for liver cancer, liver tumors that used to be in the restricted area of ​​surgery can also be safely surgically resected. Therefore, the resection rate of liver tumors has been significantly improved, coupled with the improvement of perioperative management techniques, the incidence of postoperative ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K48/00A61P35/00
Inventor 杨连粤秦承东
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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