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Non-enzyme electrochemical transducer for detecting glucose

An electrochemical and sensor technology, applied in the field of electrochemical sensor preparation, can solve the problems of affecting sensor selectivity, and it is not easy for glucose oxidase to be fixed on electrodes for a long time, and achieve fast electron transfer rate, good analytical performance, and high sensitivity. Effect

Active Publication Date: 2014-12-24
广州衡创测试技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the oxidation of hydrogen peroxide needs to be carried out at a higher potential, and many electroactive substances under high potential conditions will be oxidized and interfered, affecting the selectivity of the sensor.
The sensitivity and selectivity of glucose sensors based on enzymatic reactions are determined by the activity of glucose oxidase, which is affected by factors such as temperature, humidity and chemical environment, and glucose oxidase is not easy to be fixed on the electrode for a long time. superior

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An electrochemical sensor for enzyme-free detection of glucose, the preparation method is as follows:

[0018] (1) Chlorauric acid (HAuCl 4 ) aqueous solution: 1g of chloroauric acid was dissolved in 160mL of deionized water, stirred at 40°C for 27min, and the stirring rate was 425r / min to obtain chloroauric acid (HAuCl 4 ) aqueous solution;

[0019] (2) Preparation of graphene (Gr) dispersion liquid: disperse 0.1g graphene into 80mL deionized water, ultrasonically disperse for 20 minutes, and 2mL chloroauric acid (HAuCl) described in step (1) 4 ) aqueous solution mixing and stirring;

[0020] (3) Preparation of graphene / nano gold (Gr-Au): graphene (Gr) and chloroauric acid (HAuCl 4 ) 50mL of the mixed solution was heated in an oil bath at a constant temperature to maintain 93°C. Under stirring at a rate of 2300r / min, 45mL of 1% trisodium citrate solution was quickly added, and the stirring was continued for 60min to obtain a purple-red liquid; the solid obtained by ...

Embodiment 2

[0024] An electrochemical sensor for enzyme-free detection of glucose, the preparation method is as follows:

[0025] (1) Chlorauric acid (HAuCl 4 ) aqueous solution: 1g of chloroauric acid was dissolved in 100mL of deionized water, stirred at 30°C for 40min, and the stirring rate was 300r / min to obtain chloroauric acid (HAuCl 4 ) aqueous solution;

[0026] (2) Preparation of graphene (Gr) dispersion liquid: disperse 0.1g graphene into 50mL deionized water, ultrasonically disperse for 20 minutes, and 0.5mL chloroauric acid (HAuCl) described in step (1) 4 ) aqueous solution mixing and stirring;

[0027] (3) Preparation of graphene / nano gold (Gr-Au): graphene (Gr) and chloroauric acid (HAuCl 4 ) 50mL of the mixed solution was heated in an oil bath at a constant temperature to maintain 92°C. Under stirring at a rate of 1500r / min, 25mL of 1% trisodium citrate solution was quickly added, and the stirring was continued for 50min to obtain a purple-red liquid; the solid obtained b...

Embodiment 3

[0031] An electrochemical sensor for enzyme-free detection of glucose, the preparation method is as follows:

[0032] (1) Chlorauric acid (HAuCl 4 ) aqueous solution preparation: 1g of chloroauric acid was dissolved in 200mL of deionized water, stirred at 50°C for 15min, and the stirring rate was 450r / min to obtain chloroauric acid (HAuCl 4 ) aqueous solution;

[0033] (2) Preparation of graphene (Gr) dispersion liquid: disperse 0.1g graphene into 100mL deionized water, ultrasonically disperse for 20 minutes, and 5mL chloroauric acid (HAuCl) described in step (1) 4 ) aqueous solution mixing and stirring;

[0034] (3) Preparation of graphene / nano gold (Gr-Au): graphene (Gr) and chloroauric acid (HAuCl 4 ) 50mL of the mixed solution was heated in an oil bath at a constant temperature to maintain 98°C. Under stirring at a rate of 3000r / min, 50mL of 1% trisodium citrate solution was quickly added, and the stirring was continued for 90min to obtain a purple-red liquid; the solid...

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Abstract

The invention relates to a non-enzyme electrochemical transducer for detecting the glucose content on the basis of graphene / nanogold modified electrode (Gr-Au / GC). According to the non-enzyme electrochemical transducer for detecting the glucose, chloroauric acid (HAuCl4) and graphene (Gr) are used for preparing a modified electrode, the prepared modified electrode is extremely highly active on oxidation of the glucose, highly dispersed nanoparticles in the modified electrode provide large superficial area for electro-catalysis reaction, the detection sensitivity is improved, the electron transfer rate is increased, and analysis performance of the glucose is improved. According to the non-enzyme electrochemical transducer for detecting the glucose, the detection sensitivity of the glucose is high, the detection limit is low, and the detection range is wide.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method of an enzyme-free electrochemical sensor for glucose detection. Background technique [0002] Sensors for glucose detection have become one of the most popular biosensors due to their importance in clinical diagnosis, pharmaceutical analysis, and food industry. [0003] The most widely used glucose sensor based on enzymatic reaction, its most basic principle is: using the immobilized glucose oxidase membrane as a recognition device to convert the detected amount of glucose into a usable output signal. The glucose sensor basically consists of an enzyme membrane and a Clark oxygen electrode or a hydrogen peroxide electrode. Under the catalysis of glucose oxidase, the oxidation reaction of glucose consumes oxygen and produces gluconolactone and hydrogen peroxide. Glucose oxidase is immobilized by the semipermeable membrane on the surface clo...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/38
Inventor 孙京华潘正海
Owner 广州衡创测试技术服务有限公司
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