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Method for preparing germanium-enriched phellinus igniarius fermentation product

A fermentation product, Phellinus linteus technology, applied in the field of bioengineering, can solve the problems of not considering special nutritional requirements, low content of effective components, lack of pertinence of components, etc., and achieve the effect of enriching metabolites

Inactive Publication Date: 2014-12-24
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The above-mentioned technologies generally only use liquid or solid-state fermentation methods, simply imitating the method of conventional edible and medicinal mycelia fermentation, using beef extract, peptone or common yeast powder as nitrogen sources, and sucrose, glucose or rice as the main carbon source , adding germanium dioxide and other inorganic germanium-based germanium sources to the medium, without considering the special nutritional requirements of specific edible and medicinal bacteria on the medium during the fermentation process, and the components of the formula lack pertinence
Due to the limited culture time of edible and medicinal fungi, inorganic germanium is generally not easily absorbed by the mycelia. Simple preparation of germanium-rich edible and medicinal fungus mycelia through liquid fermentation with limited culture time will lead to organic The content of effective components such as germanium and germanium polysaccharides is not high

Method used

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  • Method for preparing germanium-enriched phellinus igniarius fermentation product
  • Method for preparing germanium-enriched phellinus igniarius fermentation product
  • Method for preparing germanium-enriched phellinus igniarius fermentation product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Material preparation

[0066]Neutral protease (enzyme activity is 200,000 U / g), amylase (enzyme activity is 100,000 U / g), pectinase (enzyme activity is 50,000 U / g), cellulase (enzyme activity is 30,000 U / g) U / g), provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0067] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0068] Preparation of exogenous additives:

[0069] a. Preparation of hemp leaf enzymatic hydrolyzate: wash the picked hemp leaves, vacuum freeze-dry, weigh 100g and add 500mL water, grind into a slurry, and sterilize at 95°C for 5min to inactivate various enzymes; adjust the slurry pH 5.0, add a complex enzyme consisting of 1g cellulase and 1g pectinase, carry out enzymatic hydrolysis reaction at 45°C for 1.0 hour, and inactivate the enzyme at 95°C for 6 minutes; then adjust the pH of the slurry to 6.0, add 1.75g ​​of The compound enzyme composed of amylase and 1.75g...

Embodiment 2

[0102] 1. Material preparation

[0103] Pectinase (enzyme activity is 30,000 U / g), neutral protease (enzyme activity is 300,000 U / g), amylase (enzyme activity is 200,000 U / g), cellulase (enzyme activity is 20,000 U / g) U / g), provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0104] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0105] Preparation of exogenous additives:

[0106] a. Preparation of hemp leaf enzymatic hydrolyzate: wash the picked hemp leaves, vacuum freeze-dry, weigh 100g and add 600mL water, grind into a slurry, sterilize at 90°C for 8min, inactivate various enzymes; adjust the slurry pH 5.5, add a compound enzyme consisting of 1.1g cellulase and 2.2g pectinase, carry out enzymatic hydrolysis reaction at 55°C for 1.5 hours, and inactivate the enzyme at 90°C for 8 minutes; then adjust the pH of the slurry to 6.5, add The compound enzyme composed of 0.56g amylase and 1.68g neu...

Embodiment 3

[0121] 1. Material preparation

[0122] Pectinase (enzyme activity is 30,000 U / g), neutral protease (enzyme activity is 200,000 U / g), amylase (enzyme activity is 100,000 U / g), cellulase (enzyme activity is 30,000 U / g) U / g), provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0123] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0124] Preparation of exogenous additives:

[0125] a. Preparation of hemp leaf enzymatic hydrolyzate: wash the picked hemp leaves, vacuum freeze-dry, weigh 100g and add 700mL water, grind into a slurry, sterilize at 92°C for 6min, inactivate various enzymes; adjust the slurry pH 4.5, add a compound enzyme composed of 1.86g cellulase and 0.62g pectinase, carry out enzymolysis reaction at 50°C for 0.5 hours, and inactivate the enzyme at 93°C for 5 minutes; then adjust the pH of the slurry to 7.0, add The compound enzyme composed of 2.4g amylase and 1.6g neutral protea...

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Abstract

The invention discloses a method for preparing a germanium-enriched phellinus igniarius fermentation product. The method comprises the following steps: inoculating activated phellinus igniarius into a liquid seed culture medium with a moringa oleifera extract and cultivating to obtain a phellinus igniarius liquid strain; firstly, inoculating the phellinus igniarius liquid strain into a solid culture medium with hemp fimble leaf zymolyte, sugar beet pulp, germanium-enriched yeast and tea seed cake and cultivating to obtain germanium-enriched phellinus igniarius solid culture I; and inoculating the phellinus igniarius liquid strain into the liquid culture medium with germanium-enriched phellinus igniarius culture I, germanium-enriched yeast and gorgonian zymolyte and cultivating to obtain the germanium-enriched phellinus igniarius liquid fermentation product. An exogenous additive beneficial to organic germanium and germanium polysaccharide metabolism is added at the stages of solid fermentation and liquid fermentation; as an exogenous additive, the culture product of phellinus igniarius at the solid fermentation stage is added to the liquid fermentation stage to be recycled; through co-cultivation of the solid fermentation stage and the liquid fermentation stage, the content and the activity of the organic germanium and phellinus igniarius germanium polysaccharide in the final phellinus igniarius fermentation product are greatly increased.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for preparing a germanium-rich Phellinus fermented product. Background technique [0002] Phellinus igniarius belongs to Basidiomycotina, Phellinus class, Rustiaceae, Pinifera. Phellinus extract has significant effects in the clinical application of inhibiting cancer cell metastasis and preventing cancer recurrence after surgery. It is currently a medicinal fungus with the highest efficiency among internationally recognized biological cancer treatment agents. As the currently internationally recognized large medicinal fungus with the best anti-cancer effect, the research on Phellinus antitumor mainly focuses on the pharmacological active ingredient Phellinus polysaccharide. [0003] Germanium is a highly biologically active trace element and one of the indispensable substances for the human body. Germanium mainly exists in the form of compounds and complexes, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/04C12R1/645
Inventor 程俊文贺亮魏海龙胡传久付立忠李海波邹景泉
Owner ZHEJIANG FORESTRY ACAD
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