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Preparation method and application of lactobacillus plantarum and bacteriocin of lactobacillus plantarum

A technology of Lactobacillus plantarum and bacteriocins, applied in the field of microorganisms, can solve the problems of high cost of bacteriocins, insufficient research on the antibacterial spectrum of bacteriocins, and restrictions on the development and utilization of bacteriocins, and achieve high antibacterial activity and antibacterial The effect of broad spectrum and simple method

Inactive Publication Date: 2014-12-24
ZHEJIANG GONGSHANG UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the current cost of preparing bacteriocins is very high, the sources of bacteriocins are insufficient, the research on the antibacterial spectrum of bacteriocins is not deep enough, and the mechanism of action of bacteriocins is not clear enough, and the above problems will greatly limit the development of bacteriocins. development and utilization

Method used

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  • Preparation method and application of lactobacillus plantarum and bacteriocin of lactobacillus plantarum
  • Preparation method and application of lactobacillus plantarum and bacteriocin of lactobacillus plantarum
  • Preparation method and application of lactobacillus plantarum and bacteriocin of lactobacillus plantarum

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Embodiment 1

[0025] A strain of Lactobacillus plantarum ( Lactobacillus plantarum ) ZJ5, characterized in that: the strain has been deposited in the General Microbiology Center of China Microbial Culture Collection Management Committee on March 29, 2013, its preservation number is CGMCC NO.7390, and the preservation address is: Beichen West Road, Chaoyang District, Beijing No. 1, No. 3, Institute of Microbiology, Chinese Academy of Sciences.

[0026] Strain identification:

[0027] Bacteriocin-producing strain: Lactobacillus plantarum ( Lactobacillus plantarum ) ZJ5. The Lactobacillus plantarum ZJ5 strain was streaked on the MRS solid medium and cultured at 30 oC for 24 h. Pick a single colony in a test tube containing 10 ml of MRS liquid medium. After standing for 24 hours, inoculate it into 100 ml of liquid medium at 3% of the inoculum, and then stand for 24 hours and store at 4 oC. The colony shape of this bacteria is round, moist, convex or flat on the surface ( figure 1 A), opaque, whit...

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Abstract

The invention relates to the technical field of microorganisms and discloses lactobacillus plantarum, bacteriocin which is generated by lactobacillus plantarum as well as a preparation method and application of lactobacillus plantarum and bacteriocin. The strain is preserved in CGMCC on 29th, March, 2013 with the preservation number of CGMCCNO.7390 and the preservation address of Institute of Microbiology, CAS, #3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The new strain of lactobacillus plantarum and the bacteriocin which is generated by lactobacillus plantarum are wide in antimicrobial spectrum and high in antimicrobial activity and can be used for inhibiting various food-borne pathogenic bacteria and Gram-positive and Gram-negative bacteria inducing food spoilage. The method for preparing bacteriocin by using lactobacillus plantarum is simple and low in cost.

Description

Technical field [0001] The invention involves the field of microbiological technology, and especially involves a plant lactobacar and its processed methods and applications produced. Background technique [0002] The source of bacterial is mainly bacteria. In a specific metabolic pathway, the protein, polypeptide or protein complex produced by ribosomes.Bacterial activity is limited to inhibiting bacteria that are closely related, but it does not have any inhibitory activity for bacteria.Although bacterialin is a antibacterial agent that suppresses food pathogenic bacteria such as Salmonella, it is different from traditional antibiotics; in the traditional sense of peptide antibiotics, its main source is formed by the catalytic of cell polymers and is not subject to specific ones.Gene encoding, but bacterialin is encoded by specific structural genes, so molecular biology can be used for transformation.The synthesis and action patterns of these bacterium are very different from th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00C12R1/25
Inventor 顾青宋达峰
Owner ZHEJIANG GONGSHANG UNIVERSITY
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