New chicken seminal fluid purifying method

A new method, semen technology, applied in the field of separation and cell culture, can solve the problems of reducing semen utilization rate, cross-infection, high sperm mortality rate, etc.

Active Publication Date: 2014-12-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, artificial insemination technology is widely used in modern poultry production, which is very easy to cause cross infection, high sperm mortality rate and reduced semen utilization

Method used

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  • New chicken seminal fluid purifying method
  • New chicken seminal fluid purifying method
  • New chicken seminal fluid purifying method

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Embodiment

[0020] The technical solution of the present invention is: a novel method for purifying chicken semen, using 19% percoll working solution to effectively separate the sperm in the semen, the specific steps are as follows:

[0021] (1) Use the "massage method" to collect 200-500ul of the semen of the normal sperm-producing Langshan chicken. After liquefaction, that is, half an hour after collection, add 3 times the volume of sperm culture solution, mix well, centrifuge at 800rpm, discard the supernatant, The sperm culture fluid is 1 part of chicken serum plus 4 parts of Earle's solution;

[0022] (2) The semen after the resuspended precipitation of the sperm culture fluid is slowly added to the 2ml concentration of the 19% percoll working solution layer along the tube wall, and the 19% percoll working solution is placed in a 5ml finger tube, Centrifuge at 800rpm for 20min, discard the supernatant, flick the bottom of the tube with your fingers to loosen the precipitate;

[0023...

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PUM

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Abstract

The invention discloses a new chicken seminal fluid purifying method; a modified sperm culture solution comprising 1 part of chicken serum and 4 parts of Earle's solution is added, separated by 19%percoll working liquid, then centrifuged in 800rpm, and cultured in a constant temperature incubator, the sperm quality can be significantly improved, and by use of the difference between the density of sperm and the density of other components in the seminal fluid and the f linear motion ability of the sperm, the seminal fluid purifying purpose can be achieved.

Description

Technical field: [0001] The invention relates to the field of animal cell culture, in particular to cell culture and separation techniques. technical background: [0002] Chicken semen consists of sperm and semen. The seminal fluid is the secretion of the seminiferous tubules, epididymis, and vas deferens, and also contains clear fluid. There are factors in the transparent fluid that improve sperm motility, respiration and glucose consumption, and promote premature aging of sperm. At present, artificial insemination technology is widely used in modern poultry production, which is very easy to cause cross-infection, high sperm mortality rate and reduced semen utilization rate. In addition, the fecundity of the chicken industry continues to decline, and about 10% of asthenozoospermia chickens are eliminated from commercial breeds and local breeds in my country every year. This has brought huge losses to production and local genetic resource protection. How to use some asthen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
Inventor 常国斌陈国宏徐琪马腾吴信生翟飞赵文明
Owner YANGZHOU UNIV
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