Living cell culture environment control system for microscope

Abstract

The invention relates to a living cell culture environment control system for a microscope. The control system comprises a host computer, a controller, an incubator and an integrated actuator, wherein the host computer can be used for setting temperature and CO2 concentration; the temperature can be separately set and controlled point by point; the incubator has a laminar flow air supply structure of which the upper layer and the lower layer independently work; a top plate of the incubator is arranged above a microscope stage and below a collecting lens turntable, and can be opened and closed towards two sides; the controller comprises a multi-point temperature controller and a CO2 concentration controller; temperature detecting probes are respectively arranged above and below the microscope stage and on a left bottom plate and a right bottom plate of the incubator; in combination with the CO2 concentration above the microscope stage, the temperature is controlled at the lower layer and the temperature and the CO2 concentration can be simultaneously controlled at the upper layer. The integrated actuator is of an integral structure with heating, humidifying and CO2 mixing functions. The living cell culture environment control system is simple in mechanism, easy to operate, and small in size, can be quickly disassembled and assembled, and has good control accuracy, and the function of the high-performance microscope is given a full play.

Description

technical field [0001] The invention relates to a living cell culture environment control system for a microscope, which belongs to the technical field of cell imaging. Background technique [0002] Live cell imaging is a research method to observe the movement changes of the internal structure of cells and the physiological process of cells through real-time dynamic or image acquisition over a period of time. On the one hand, live cell imaging needs to maintain the physiological activity of cells, and on the other hand, it can use a microscope for real-time observation and image acquisition, so it needs the support of a cell culture environment control system. A cell culture environment control system is an essential component for live cell imaging. In the absence of medium or suitable temperature and other conditions, animal cells can usually only maintain normal activities for a short period of time. Therefore, to achieve live cell imaging on a microscope, it is necessa...

AI Technical Summary

Problems solved by technology

The first type of system is small in size, and the biggest problem is that the accuracy of temperature control is not enough; secondly, due to the temperature difference between the inside and outside of the culture chamber cover, there will be condensed water, which seriously affects the observation effect of DIC and transmitted light; thirdly, due to the The upper cover of the cultivation room has temperature control wiring, and the shadow will affect the effect of multi-point shooting

The second type of system, due to its large size, heavy weight, and complicated installation, is generally suitable for a living cell workstation system with specific functions. If not, it needs to be disassembled frequently, and this kind of operation has a great risk of damaging the box or the instrument

[0004] In summary, the above two commonly used living cell environment control systems are subject to certain limitations in application adaptability due to their respective structures.

Images