Clostridium beijerinckii for hydrogen generation via fermentation as well as fermentation method and application of clostridium beijerinckii

Abstract

The invention discloses clostridium beijerinckii for hydrogen generation via fermentation as well as a fermentation method and an application of the clostridium beijerinckii. The clostridium beijerinckii is preserved in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM), and has the preservation number of CGMCC No.9411. The culture method of the clostridium beijerinckii comprises the following steps: firstly, carrying out anaerobic culture on a freeze-stored liquid of the clostridium beijerinckii so as to obtain a cultured bacterial culture solution; centrifuging the bacterial culture solution and then resuspending thalli so as to obtain a resuspended bacterial solution taken as an inoculant source; inoculating the inoculant source into a hydrogen generation culture solution and carrying out photophobic culture on a constant-temperature shaking water bath for generating hydrogen until the hydrogen generation is finished. The clostridium beijerinckii can be fermented to generate hydrogen by utilizing a carbon source and a nitrogen source which are common in natural world and are difficultly utilized by the majority of microorganisms, and ethyl alcohol, butyl alcohol and other biofuels are generated while the hydrogen generation is carried out via the fermentation. The clostridium beijerinckii disclosed by the invention is the first novel multifunctional clostridium beijerinckii strain in the current report, and can be applied to the hydrogen generation of biomass via fermentation, the production of biofuels such as ethyl alcohol and butyl alcohol. Thus, the clostridium beijerinckii disclosed by the invention has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes and relates to a new Clostridium beijerinckii, in particular to a Clostridium beijerinckii used for fermentative hydrogen production and a fermentation method and application thereof. Background technique [0002] Clostridium beijejerinckii is a group of unicellular prokaryotic microorganisms that can grow in a sugar-rich environment, strictly anaerobic, and convert monomeric sugars into hydrogen and carbon dioxide in an anaerobic environment. [0003] Hydrogen is a kind of clean energy, and the biological fermentation hydrogen production method has greater potential than the chemical hydrogen production method because its production process is more energy-efficient. Biofermentation hydrogen production can convert a variety of biomass, such as wood, crop straw, industrial wastewater, and cellulose-containing solid waste into hydrogen. However, biomass contains a large amount of cellulose, hemice...

AI Technical Summary

Problems solved by technology

However, many bacteria cannot effectively convert various substrates to ferment hydrogen, which has become a limiting factor in biohydrogen production.

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