Micro-fluidic chip magnetic-bead chaotic mixing method and apparatus

Abstract

The invention discloses a micro-fluidic chip magnetic-bead chaotic mixing method and an apparatus. The micro-fluidic chip magnetic-bead chaotic mixing apparatus comprises a spherical frame formed by six same circular frames which are successively at intervals with the pairwise interval included angle of 30 DEG along the spherical longitude direction, the middle in the spherical frame is provided with a micro-fluidic chip, and three pieces of radial electromagnet are uniformly distributed along the circumferential direction at the external of each circular frame. A microfluid solution is introduced into a microfluid inlet channel, and a magnetic-bead solution is introduced into a magnetic-bead solution inlet channel at the same time, the three pieces of electromagnet on a first circular frame are powered on with UVW low-frequency three-phase alternating current, so that magnetic beads in a mixed solution form a magnetic-bead chain, and the magnetic-bead chain continuously rotates in the solution along with variation of the magnetic field, by increasing the frequency of the three-phase alternating current on the three pieces of electromagnet on the first circular frame, the magnetic-bead chain is fractured, and the three pieces of electromagnet on each of a second circular frame to a sixth circular frame are successively powdered on. Stirring is realized through sequential control on a space rotating field with spherical layout, and the solution is guaranteed to be completely mixed.

Description

technical field [0001] The invention relates to solution mixing technology, in particular to a chaotic mixing method and device for a microfluidic chip, which is used to improve the liquid mixing efficiency in the microfluidic chip. Background technique [0002] Mixing is a physical process, the most basic and necessary operation of any chemical and biochemical reaction, and the effect of mixing depends on the mixing process and method. In macroscopic systems, mixing is usually accomplished by convection. In microfluidic chips, the structural size of the system is usually smaller than hundreds of microns. The Reynolds coefficient of the fluid at the micron scale is very small, and turbulent mixing cannot occur. The fluid flows in a completely laminar flow state, and the mixing can only be carried out by diffusion. However, Effective and rapid mixing is difficult to achieve by diffusion, especially for fluids with fast flow rates or soluble substances with low diffusion coef...

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Problems solved by technology

[0004] There is an existing micro-mixer that uses an external rotating magnetic field to drive a micro-magnetic stirring bar for mixing. The vortex is formed by the stirring of the micro-stirring bar, thereby greatly improving the mixing efficiency, but its structure is relatively complicated, and the middle of the micron-scale stirring b

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