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A mucus and airway technology, applied in the field of biomedicine, can solve the problems of not meeting the needs of experimental research and small sample capacity.
Inactive Publication Date: 2014-11-19
CHANGZHOU UNIV
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Problems solved by technology
Another way is to culture human airway epithelial cells, let them naturally secrete airway mucus and t
Method used
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example 1
[0008] 1. Add calcium chloride, sodium chloride, sodium hydrogen phosphate, and sodium dihydrogen phosphate to 10ml of deionized water to prepare a buffer.
[0009] 2. Add 0.1 g of DPPC to the phosphate buffer solution prepared above, and stir magnetically for 2 hours.
[0010] 3. Add 0.12g of HEC and 0.11g of BSA into the reaction solution and mix for 5 days with a vertical mixer.
[0011] 4. Add 1ml of GA solution cross-linking agent into the mixed solution to induce cross-linking reaction at 4°C.
[0012] 5. The solution after the cross-linking reaction was collected and freeze-dried.
example 2
[0014] 1. Add calcium chloride, sodium chloride, sodium hydrogen phosphate, and sodium dihydrogen phosphate to 10ml of deionized water to prepare a buffer.
[0015] 2. Add 0.1 g of DPPC to the phosphate buffer solution prepared above, and stir magnetically for 2 hours.
[0016] 3. Add 0.24g of HEC and 0.11g of BSA to the reaction solution and mix for 5 days with a vertical mixer.
[0017] 4. Add 1ml of GA solution cross-linking agent into the mixed solution to induce cross-linking reaction at 4°C.
[0018] 5. The solution after the cross-linking reaction was collected and freeze-dried.
example 3
[0020] 1. Add calcium chloride, sodium chloride, sodium hydrogen phosphate, and sodium dihydrogen phosphate to 10ml of deionized water to prepare a buffer.
[0021] 2. Add 0.1 g of DPPC to the phosphate buffer solution prepared above, and stir magnetically for 2 hours.
[0022] 3. Add 0.68g of HEC and 0.11g of BSA to the reaction solution and mix for 5 days with a vertical mixer.
[0023] 4. Add 1ml of GA solution cross-linking agent into the mixed solution to induce cross-linking reaction at 4°C.
[0024] 5. The solution after the cross-linking reaction was collected and freeze-dried.
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PUM
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Abstract
The invention aims to provide a preparation method of an airway mucus model. The chemical components and physical property of the mucus model are matched with the chemical components and physical property of airway mucus of a human body. According to a formula, the model comprises dipalmitoylphosphatidylcholine (DPPC), hydroxyethyl cellulose (HEC), blood serum albumin (BSA), glutaraldehyde (GA) and a phosphate buffer solution (PBS). The preparation method comprises the steps of (1) preparing the PBS as required; (2) dissolving DPPC into the prepared PBS, and magnetically stirring for 2h; (3) adding the BSA and the HEC into the stirred solution, and mixing the solution for several days by using a mixer; and (4) adding GA into the solution prepared in the step (3), carrying out corsslinking reaction within a certain temperature range, collecting the crosslinked solution, and carrying out freeze drying. The preparation method is simple in process, low in cost and nonhazardous; and the biological, physical and chemical properties of the mucus model are matched with the biological, physical and chemical properties of a human airway mucus model, and therefore, the airway mucus model plays a great important role on the aspects of mucus protection mechanism, pathologic analysis, drug delivery and genetic vector therapy.
Description
technical field [0001] The invention relates to a synthetic airway mucus model synthesis technology, in particular to an airway mucus used in the field of biomedicine and a synthesis method thereof. Background technique [0002] Respiratory mucus is an important part of the airway defense barrier. The mucus layer can adhere to the particles of pathogenic microorganisms and harmful substances contained in inhaled air, and expel them through ciliary movement and coughing. In addition, it also plays an important role in maintaining airway humidification and assisting epithelial cell functions. But if mucus production is abnormal, chronic and excessive mucus secretion (also known as chronic mucus hypersecretion) accumulates, which can lead to respiratory disease. Therefore, it is very important to understand the physiological, chemical and physical properties of airway mucus. In existing studies, the collection of airway mucus is difficult. The mucus of patients with respirat...
Claims
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