Preparation method of MS medium

Abstract

The invention relates to a preparation method of an MS medium. The MS medium comprises macro-elements (a mother liquor 1), trace elements (a mother liquor 2), iron salt (a mother liquor 3) and organic components (a mother liquor 4). The preparation method is characterized in that Ca<2+> in the macro-elements is individually processed in the preparation process of the mother liquor 1 to prepare a storage liquor; in the preparation process of the mother liquor 2, sodium molybdate (NaMoO4.2H2O) is individually dissolved by distilled water at room temperature, then the dissolved sodium molybdate is poured into the trace element mother liquor 2; and in the preparation process of the mother liquor 3, FeSO4.7H2O and Na2-EDTA.2H2O are accurately weighed according to the consumption of 1000ml of the mother liquor, are respectively placed in a beaker with 500ml of distilled water, are heated and are continuously stirred to dissolve, heating is stopped until the solution approaches boiling, the obtained solutions are cooled, the cooled solutions are poured into reagent bottles, and the reagent bottles are put in a refrigerator for preservation. The preparation method of the MS medium solves the problems of difficult dissolving of sodium molybdate in the medium mother liquor preparation process and the precipitate generation or crystal precipitation in the mother liquor preparation and storage processes.

Description

technical field [0001] The invention relates to the field of medium preparation, in particular to a preparation method of MS medium. Background technique [0002] MS medium was designed by Murashige and Skoog for tobacco cell culture in 1962. It is characterized by high concentration of inorganic salts and ions. It is a relatively stable ion balance solution. It has high nitrate content and the quantity and ratio of nutrients are appropriate. It can meet the nutritional and physiological needs of plant cells, so it has a wide range of applications. It is used as the basic medium for the rapid propagation of most plant tissue cultures. [0003] The composition of MS medium is (mg / L): macroelement: NH 4 NO 3 、KNO 3 , CaCl 2 2H 2 O, MgSO 4 ·7H 2 O. KH 2 PO 4 ; Trace elements: KI, H 3 BO 3 , MnSO 4 4H 2 O, ZnSO 4 ·7H 2 O, Na 2 MoO 4 2H 2 O, CuSO 4 ·5H 2 O, CoCl 2 ·6H 2 O; iron salt: FeSO 4 ·7H 2 O, Na 2 -EDTA·2H 2 O; Organic ingredients: inositol, niaci...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of MS medium, which can solve the problem that sodium molybdate is difficult to dissolve when the mother liquor of the culture medium is prepared, and precipitation or crystallization occurs during the preparation and storage of the mother liquor.