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Tissue culture rapid propagation method for Mucuna bracteata DC. ex Kurz

A technology of rapid propagation of quinoa bean and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low germination rate, difficult collection of quinoa bean seeds, and increased demand

Inactive Publication Date: 2014-11-12
广东农垦热带作物科学研究所
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the seeds of quinoa bean are not easy to collect, and the germination rate is low under natural conditions, and the demand for its seedlings in current production is increasing sharply. By adopting biotechnology means, a kind of tissue culture of quinoa bean is provided Rapid propagation method to establish a high-efficiency quinoa bean tissue culture rapid propagation technology system

Method used

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Examples

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Effect test

Embodiment

[0012] 1) Collection and disinfection of quinoa bean explants: collect mature and healthy seeds of quinoa bean, wash them with clean water and place them on an ultra-clean workbench. 0.1% HgCl 2 Soak and sterilize in aqueous solution for 15 minutes, then rinse with sterile water for 5 times, blot the surface moisture with sterile filter paper, use a scalpel to gently draw a small opening on the back of the seed, and inoculate it with 30g / L sucrose and 6.5g / L agar , pH is 5.8, and has been placed at a temperature of 121°C and a pressure of 1.1 kg·cm -2 Sterilize on MS medium for 20 min under conditions. After inoculation, place it at a temperature of 27°C±1°C, the light source is a fluorescent lamp, the illuminance is 2000-3000 lx, and the light time is 12 h·d - 1 cultured under the conditions;

[0013] 2) Induction and multiplication of clustered buds of quinoa bean: after 4 days of cultivation, the radicle protrudes from the seeds, and after 10 days, the epicotyl elongates...

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Abstract

The invention belongs to the field of biotechnology and particularly relates to a tissue culture rapid propagation method for Mucuna bracteata DC. ex Kurz. The method includes the steps that Mucuna bracteata DC. ex Kurz explants are collected and disinfected; Mucuna bracteata DC. ex Kurz cluster buds are induced and propagated; rooting culture is performed on strong seedlings of Mucuna bracteata DC. ex Kurz; Mucuna bracteata DC. ex Kurz seedlings are hardened and transplanted. The method can effectively solve the problems that Mucuna bracteata DC. ex Kurz seeds are hard to collect, and the rate of emergence of the seeds is low under natural conditions, and the increasing market demands of interplanting leguminous green manure for building ecological rubber plantations, oil palm plantations and orchards in the modern agricultural development process for seedlings can be met.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for tissue culture and rapid propagation of quinoa bean. Background technique [0002] quinoa beans ( Mucuna bracteata DC. ex Kurz), belonging to Leguminosae (Leguminosae) Mucuna perennial twining vines, born in forests or grasslands, hillsides, roadsides or streams at an altitude of 600-2000 meters, mainly distributed in Malaysia, Myanmar, Thailand, Vietnam and other Southeast Asian countries, my country's Yunnan and Hainan also have sporadic distribution. Its parasitic rhizobium has the function of biological nitrogen fixation, and plays an important role in enriching soil nutrients, improving soil physical properties, and improving soil water and fertilizer retention performance. beans as a cover crop. [0003] In recent years, with the development of our country's economy and society, the traditional agricultural planting and farming models can no longer m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张全琪倪燕妹郑杰陈海坚黄志王力前
Owner 广东农垦热带作物科学研究所
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