Tissue culture rapid propagation method for Mucuna bracteata DC. ex Kurz
A technology of rapid propagation of quinoa bean and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low germination rate, difficult collection of quinoa bean seeds, and increased demand
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[0012] 1) Collection and disinfection of quinoa bean explants: collect mature and healthy seeds of quinoa bean, wash them with clean water and place them on an ultra-clean workbench. 0.1% HgCl 2 Soak and sterilize in aqueous solution for 15 minutes, then rinse with sterile water for 5 times, blot the surface moisture with sterile filter paper, use a scalpel to gently draw a small opening on the back of the seed, and inoculate it with 30g / L sucrose and 6.5g / L agar , pH is 5.8, and has been placed at a temperature of 121°C and a pressure of 1.1 kg·cm -2 Sterilize on MS medium for 20 min under conditions. After inoculation, place it at a temperature of 27°C±1°C, the light source is a fluorescent lamp, the illuminance is 2000-3000 lx, and the light time is 12 h·d - 1 cultured under the conditions;
[0013] 2) Induction and multiplication of clustered buds of quinoa bean: after 4 days of cultivation, the radicle protrudes from the seeds, and after 10 days, the epicotyl elongates...
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