Compound cultivation method of Chinese medicinal material Bletilla striata interplanted in pine forest
A cultivation method and technology of traditional Chinese medicinal materials, which are applied in the field of medicinal plant seedling raising engineering, can solve the problems of difficulty in breeding Bletilla striata inoculum, high technical level requirements, no popularization and application, etc., so as to improve forest coverage, improve stand conditions, The effect of raising the level of cultivation
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Embodiment 1
[0026] The present invention is a method for compound cultivation of Chinese medicinal material Bletilla striata interplanted in pine forests, characterized in that: the cultivation method comprises the following steps:
[0027] (1) Tissue culture and sowing: prepare nutrient base, the nutrient base is 1 / 2MS plus 100g / L mashed potato plus NAA0.5mg / L plus 6g agar plus 30g sucrose, after the nutrient base is prepared, it will be ready at the end of December Sowing is carried out, and after germination and rooting, the seedlings are moved to the transfer tray for hardening in January-February of the following year;
[0028] (2) Seedling hardening with translocation tray: The translocation tray is made of 40% light substrate plus 60% loose soil. The tissue culture seedlings transplanted in January-February will be transplanted into pine forest after new roots germinate in March-April. Down;
[0029] (3) Soil preparation and fertilization: Select a pine forest with a light transmi...
Embodiment 2
[0033] The present invention is a method for compound cultivation of Chinese medicinal material Bletilla striata interplanted in pine forests, characterized in that: the cultivation method comprises the following steps:
[0034] (1) Tissue culture and sowing: Prepare the nutrient base, the nutrient base is 1 / 2MS plus 80g / L mashed potatoes plus NAA0.5mg / L plus 4g agar plus 25g sucrose, after the nutrient base is prepared, it will be at the end of December Sowing is carried out, and after germination and rooting, the seedlings are moved to the transfer tray for hardening in January-February of the following year;
[0035] (2) Seedling hardening with translocation tray: The translocation tray is made of 40% light substrate plus 60% loose soil. The tissue culture seedlings transplanted in January-February will be transplanted into pine forest after new roots germinate in March-April. Down;
[0036] (3) Soil preparation and fertilization: Select a pine forest with a light transmit...
Embodiment 3
[0040] The present invention is a method for compound cultivation of Chinese medicinal material Bletilla striata interplanted in pine forests, characterized in that: the cultivation method comprises the following steps:
[0041] (1) Tissue culture and seeding: prepare nutrient base, the nutrient base is 1 / 2MS plus 90g / L mashed potatoes plus NAA0.3mg / L plus 6g agar plus 30g sucrose, after the nutrient base is prepared, bottle 120 After sterilizing by high-pressure steam for 20 minutes, cool down for standby, sow at the end of December, and move to the transplanting tray for seedling hardening in January-February of the next year after germination and rooting;
[0042] (2) Seedling hardening with translocation tray: The translocation tray is made of 40% light substrate plus 60% loose soil. The tissue culture seedlings transplanted in January-February will be transplanted into pine forest after new roots germinate in March-April. Down;
[0043] (3) Soil preparation and fertiliza...
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