Phosphorylated peptide adsorption enrichment method, additive and application
A technology for phosphorylated peptides and additives, applied in the field of protein and peptide enrichment and purification, can solve problems such as poor effect, affecting the accuracy and stability of phosphorylated peptides, and achieve increased adsorption selectivity, accuracy and stability. , reduce the effect of adsorption
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[0040] In this example, mouse liver samples were taken as the research object, and phosphorylated peptides were enriched by the titanium dioxide enrichment method. Specific enrichment methods include:
[0041] First, glycolic acid, a routinely used non-specific adsorption inhibitor, was added to the loading buffer. At the same time, part of the sample to be tested extracted from the mouse liver was dissolved in the loading buffer, and the phosphorylated Enrich the peptide; collect the enriched product, analyze it, calculate the abundance of 20 amino acids in the non-phosphorylated peptide as the first abundance, and calculate the abundance of the 20 amino acids in the protein where the non-phosphorylated peptide is located as the For the second abundance, calculate the ratio of the first abundance to the second abundance, and select at least one amino acid with a higher ratio as an additive; then add the obtained additive to the loading buffer, and use the loading buffer with ...
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