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Efficient cell slide immunohistochemical method

A technology of immunohistochemistry and cells, which is applied in the field of immunohistochemistry of cell slides, can solve the problems that it is difficult to handle large-scale, and achieve the effect of consistent experimental conditions and standards, easy control, large number of samples, and reduced number

Inactive Publication Date: 2014-10-15
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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Problems solved by technology

[0003] Conventional cell slide immunohistochemical detection is to stain a single cell slide piece by piece. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult to perform large-scale, multi-sample and multi-group testing. Medical Experimental Scientific Research

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Embodiment Construction

[0052] A high-efficiency cell-mounted immunohistochemical method. Put the sterilized glass slides in the culture dish, inoculate the cells in the culture dish for cell-mounting, and perform immunocytochemical staining after the cell culture Identification; the petri dish is a transparent material, equipped with multiple cell culture tanks of the same size that can hold glass slides, the depth of the tank is ≥ 20ml, and a glass slide can be placed in each cell culture tank; one of the culture dishes The angle is in the form of an inclined plane, which is convenient for orientation identification; the petri dish cover and the bottom of the petri dish have anastomotic dykes interlaced with each other, and the interlaced depth is ≥ 10 mm.

[0053] The specifications of the cell culture tank are 80×30 / mm (for a single slide); 64×28 / mm (for a single 60×24 cover glass); 54×28 / mm (for a single 50×24 cover glass); 38 × 28 / mm (put a single cover glass of 34 × 24 / mm); the immunocytochemi...

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Abstract

The invention discloses an efficient cell slide immunohistochemical method. The method comprises the steps as follows: large-area glass slides and a culture dish which are subjected to sterilization disinfection treatment are matched for use, the glass slides are placed in the culture dish, cells are inoculated in the culture dish for cell slide, and immune cell histochemical staining identification is performed after cell culture is finished; and the immune cell histochemical staining identification comprises the steps as follows: a tool seal is used for dipping printing oil, a rubbing surface of the tool seal is covered with a cell slide surface of each glass slide, the glass slide is pressed to rub a cell staining area which has the same size with the rubbing surface of the tool seal and consists of printing ink frame strips, and if an area is short of printing ink, the area is filled up by a printing ink coating pen. The efficient cell slide immunohistochemical method can be used for large-scale and multi-sample medical scientific experiment and research.

Description

technical field [0001] The invention relates to a method for immunohistochemistry of cell slides. Background technique [0002] Cell slide immunohistochemistry is the use of labeled specific antibodies to conduct in situ qualitative, localization or quantitative research on the distribution and content of certain chemical components in cell samples. This technique is called immunocytochemistry (immunocytochemistry) technique . [0003] Routine cell slide immunohistochemical detection is to stain a single cell slide piece by piece. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult to perform large-scale, multi-sample and multi-group testing. Medical experiment scientific research. Contents of the invention [0004] The purpose of the present invention is to provide a high-efficiency cell-mounted immunohistochemical method capable of carrying out large-scale, multi-sample and multi-group medical experim...

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Application Information

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IPC IPC(8): G01N33/532
CPCG01N33/5302G01N33/583
Inventor 鄂裘恺胡义扬郭绍文王敏燕韩志宏
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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