Penaeus monodon cell cycle protein E gene sequence and preparation method and application thereof

Abstract

The invention discloses a penaeus monodon cell cycle protein E gene sequence and a preparation method and application thereof and aims to provide the penaeus monodon cell cycle protein E gene sequence which can serve as a target site to design medicines to treat the tumors or adjust ovary development process of the penaeus monodon, and the sequence is conducive to artificial propagation of penaeus monodon cells; the nucleotide of the H gene sequence is expressed as SEQ ID NO:1; the preparation method comprises the following steps: total RNA of the penaeus monodon is extracted to synthesize a cDNA first chain; the synthesized first chain cDNA is adopted as a template; then a descending PCR method is adopted to use an adaptor primer, cycH-F1, cycH-F2 for 3' tail end PCR amplification of a target gene for twice; then the penaeus monodon cell cycle protein E gene is measured; lastly, the distribution and expression of the penaeus monodon cell cycle protein E gene are measured. The invention belongs to the field of gene clone in molecular biology.

Description

technical field [0001] The present invention relates to a cyclin H gene sequence, in particular to a cyclin H gene sequence of Penaeus monodon, the invention also relates to a preparation method of the cyclin H gene sequence of Penaeus monodon, which belongs to molecular The field of gene cloning in biology. Background technique [0002] Cell cycle regulation is a precisely regulated process. In the process of cell division, the cell cycle of eukaryotes can be summarized into two major stages: interphase of cell division (i.e. G1 period, S period and G2 period) and cell division period (i.e. M period). During the cell division period, Many cycle proteins are required to participate in the regulation to ensure its precision. [0003] As an important part of CAK, cyclin H gene is highly conserved from yeast to human. The activation of CAK requires the combination of cyclin H gene and CDK7 and MAT1, and its kinase activity is realized through the phosphorylation of CAK. Cyc...

AI Technical Summary

Problems solved by technology

[0004] For a long time, the problem of artificial breeding of Penaeus monodon has been a technical bottleneck restricting the development of the industry of Penaeus monodon

As a common method to promote the synchronous maturation of broodstock gonads, eye stalk resection is very destructive to broodstock itself, which is not conducive to sustainable breeding and increases the cost of breeding

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