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Method for rapidly propagating adiantum soboliferum

A Pteridophyte, fast technique, applied in horticultural methods, botanical equipment and methods, plant cells, etc.

Active Publication Date: 2014-10-08
WUHU OUBIAO AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the prior art, there is no report of obtaining regenerated plants of Adiantum pterygium through the callus approach

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Selection of explants: get 2cm long rhizome tip, remove impurities and appendages such as fluff around the tip;

[0034] 2) Disinfection of explants: put the above-mentioned stem tip into a 20-25KHz ultrasonic cleaner filled with tap water, keep the temperature at 30-35°C for 30min, then rinse with tap water for 10min; % alcohol for 25 seconds, treated in 0.02% mercuric solution for 5 minutes, then rinsed with sterile water 4 times, and placed in a sterile petri dish for later use;

[0035] 3) Initial induction culture: Cut off 2-3 mm at both ends of the sterilized explants, and insert the shoot tip downwards into the initial induction medium for culture. The culture conditions are light intensity 1000-2500Lx, photoperiod 5-8h / d, temperature 25°C±2°C; after 6 days of culture, transfer the shoot tip of the uncontaminated explants to a new initial induction medium, and continue to cultivate for 18d until new shoots grow; the initial induction medium The composition i...

Embodiment 2

[0042] 1) Selection of explants: get 2cm long rhizome tip, remove impurities and appendages such as fluff around the tip;

[0043] 2) Disinfection of explants: put the above-mentioned stem tip into a 20-25KHz ultrasonic cleaning machine filled with tap water and keep the temperature at 35°C for 45 minutes, then rinse with tap water for 20 minutes; Alcohol disinfection for 35 seconds, treatment in 0.02% mercuric solution for 7 minutes, then rinsed with sterile water 5 times, and placed in a sterile petri dish for later use;

[0044] 3) Initial induction culture: Cut off 2-3 mm at both ends of the sterilized explants, and insert the shoot tip downwards into the initial induction medium for culture. The culture conditions are light intensity 1000-2500Lx, photoperiod 5-8h / d, temperature 25°C±2°C; after 4 days of culture, transfer the uncontaminated explant shoot tip to a new initial induction medium, and continue to cultivate for 12d until new shoots grow; the initial induction m...

Embodiment 3

[0051] 1) Selection of explants: get 2cm long rhizome tip, remove impurities and appendages such as fluff around the tip;

[0052] 2) Disinfection of explants: put the above-mentioned stem tip into a 20-25KHz ultrasonic cleaning machine filled with tap water, keep the temperature at 30-35°C for 45min, then rinse with tap water for 20min; Alcohol disinfection for 35 seconds, treatment in 0.02% mercuric solution for 7 minutes, then rinsed with sterile water 5 times, and placed in a sterile petri dish for later use;

[0053] 3) Initial induction culture: Cut off 2-3 mm at both ends of the sterilized explants, and insert the shoot tip downwards into the initial induction medium for culture. The culture conditions are light intensity 1000-2500Lx, photoperiod 5-8h / d, temperature 25°C±2°C; after 5 days of culture, transfer the uncontaminated explant shoot tips to a new initial induction medium, and continue to cultivate for 15d until new shoots grow; the initial induction medium Co...

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PUM

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Abstract

The invention relates to a method for rapidly propagating adiantum soboliferum. The method comprises the following steps: (1) selecting an explant; (2) disinfecting the explant; (3) initially inducing and culturing; (4) carrying out callus induction; (5) carrying out proliferation culture; (6) carrying out rooting culture; and (7) acclimatizing and transplanting. The method is used for propagating adiantum soboliferum so that the production cost can be reduced, and the propagation speed and the survival rate are increased.

Description

technical field [0001] The invention relates to the technical field of rapid propagation of plants, in particular to the technical field of rapid propagation of Adiantum pterygium. Background technique [0002] Ferns are a large group of plants between bryophytes and seed plants, with a unique life history. There are about 12,000 species of ferns in the world, and my country is one of the regions with the richest fern resources in the world, with more than 2,000 species. Ferns are widely distributed, growing from mountains to seashores, from frigid to tropical regions, and their ecological types are extremely diverse, mainly native, rocky or epiphytic, and a few are aquatic or marsh. [0003] Ferns have a certain importance in the economy and are often used as raw materials for medicine, food, indicator plants, and industry and agriculture. [0004] Most ferns have high ornamental value. Although they do not have bright and eye-catching flowers and fruits, they are pleasin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00C12N5/04
Inventor 戴勤王冬梅李慧珍
Owner WUHU OUBIAO AGRI DEV
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