Reproductive method of intelligent rice sterile line

Abstract

The invention discloses a reproductive technique of an intelligent rice sterile line. The reproductive technique is adopted to achieve the effect that reproduced seeds of the intelligent rice sterile line are not required to be subjected to fluorescent sorting. In the method, the intelligent rice sterile line is used as a maintainer line and is hybridized with the corresponding rice sterile line; pollen grains carrying transgenes of the intelligent rice sterile line are aborted and cannot be fertilized, pollen grains without the transgenes are normally fertilized with female gametes of the sterile line and are fruited, and therefore, the fruited seeds of the sterile line are all genetically-modified sterile line seeds and can be used for producing hybrid rice seeds. In the method, the fluorescent sorting procedure is not required in the intelligent rice sterile line reproductive process, and only the reproduction of the maintainer line requires the fluorescent sorting, and therefore, the reproductive process of the sterile line is simplified, the reproductive efficiency of the sterile line is improved and the seed industry benefit is increased.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology and discloses a breeding method of an intelligent sterile line of rice. Background technique [0002] At present, the planting area of ​​hybrid rice in China accounts for about 50%-60% of the total planting area of ​​rice. Among them, 80% of indica rice is a hybrid rice variety, which is mainly planted in southern China and has made a great contribution to ensuring food security in my country. However, hybrid rice also There are some problems that have not been solved for a long time, such as the fertility stability of sterile lines, including three-line male sterile lines induced by high temperature to induce pollen fertility, and two-line male sterile lines induced by low temperature to be fertile, which poses a threat to the safety of hybrid seed production. Risks also include that the restorer spectrum of the three-line male sterile line is not wide, and only about 5% of the lines can...

AI Technical Summary

Problems solved by technology

[0004] There is a problem of fluorescence sorting in the breeding techniques of the above-mentioned recessive male sterile materials

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