Blood plasma specific fragment free DNA (deoxyribonucleic acid) quantitative detection kit

Abstract

The invention discloses a blood plasma specific fragment free DNA (deoxyribonucleic acid) quantitative detection kit comprising the following components: two primer pairs for amplifying TP53 gene fragments of which the lengths are respectively 400bp and 150bp, a primer pair for amplifying a reference gene Beta-actin, and fetal genome DNA, wherein the two primer pairs are consistent in backward primer sites, and the lengths of the amplified TP53 gene fragments are determined by virtue of a forward primer. According to the kit disclosed by the invention, a calibration system is introduced based on a fluorescent quantitative PCR (polymerase chain reaction) technology, the fetal genome DNA is used as background DNA, a house-keeping gene Beta-actin is used as the reference gene, a cancer suppressor gene TP53 is used as a target gene, an amplification primer is designed aiming at different lengths of fragments of the cancer suppressor gene TP53, different lengths of the fragments of the Beta-actin gene and the TP53 gene are synchronously amplified by adopting a real-time fluorescent quantitative PCR process, and the content of free DNA with specific fragment sizes in blood plasma can be quantitatively detected.

Description

technical field [0001] The invention belongs to the technical field of analysis and relates to a DNA detection kit. Background technique [0002] Cell-free DNA in peripheral blood refers to the partially degraded endogenous DNA in the peripheral blood that is free from the cells. There are three main sources: apoptosis, necrosis, and secretion. [0003] In 1989, STROUN et al. found that the free DNA in the blood of tumor patients had some characteristics of tumor cell DNA, and thus proposed that the free DNA in the blood of tumor patients may be derived from tumor cells. Five years later, the researchers detected cancer gene mutations in the plasma and serum of tumor patients, and after spectral analysis, they found that they were consistent with the primary tumor. It has been confirmed that in the blood of tumor patients, the DNA with tumor biological characteristics is mainly derived from the natural distribution of tumor cells after necrosis and apoptosis or automatic r...

AI Technical Summary

Problems solved by technology

Although it has been reported in the literature that a certain housekeeping gene in the human genome can be detected by real-time fluorescence quantitative detection technology to quantitatively detect free DNA in peripheral blood, but due to the various sizes of DNA fragments in peripheral blood, this detection technology cannot be obtained. Information about one of the DNA fragments of a specific size

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