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Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof

A susceptibility gene and mutation detection technology, applied in the field of medical molecular biology, to achieve the effect of ensuring accuracy, meeting the needs of scientific research and clinical testing, and simple operation

Inactive Publication Date: 2014-09-03
杭州美中疾病基因研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process will take a lot of time for experimental technicians and errors caused by cumbersome steps

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  • Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof
  • Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof
  • Breast carcinoma gene BRCA1/BRCA2 mutation detection kit and preparation method thereof

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Embodiment Construction

[0043] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below with reference to examples. It should be understood that the examples described here are only used to explain the present invention, and are not intended to limit the present invention.

[0044] In the present invention, in the common PCR reaction liquid system, three site primers that have been designed and verified are respectively added to prepare a premixed liquid, and each tube is marked. Then use the same template to test the performance of the master mix at different time points. Specific steps are as follows:

[0045] (1) DNA extraction from peripheral blood of volunteers with a family history of breast cancer: (using LIFE Science Whole Biood DNA Mini KIT)

[0046] 1. Take a clean 1.5ml EP tube, add 500μl Buffer AC1, then add 200μl blood sample, mix and shake for 30s

[0047] 2. Add 100μl Buffer AC2...

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Abstract

The invention discloses a breast carcinoma gene BRCA1 / BRCA2 mutation detection kit. The kit includes three primer pairs for specific amplification of BRCA1 / BRCA2 gene targeting sequences; a primer pair1 is F:GGTTGGCAGCAATATGTG; and R:AGAGGCAGAGTGGATGGA; a primer pair2 is: F:CTCTTATCCTGATGGGTTGT; and R:TCTCATCTGCTTAAAGTCCC; a primer pair 3 is: F:GGCAGGTTGTTACGAGGCAT; and R: GTGAGCTGGTCTGAATGTTCG. The produced fragments of the three primer pairs are more appropriate in size, the accuracy of direct sequencing of a PCR amplified product can be ensured. The kit has the advantages of simple operation, a site sequence can be amplified only by adding a template on a PCR instrument to obtain a specific product, and the kit is convenient and quick.

Description

technical field [0001] The invention belongs to the field of medical molecular biology and relates to a kit, in particular to a kit for detecting hot spot mutations of breast cancer susceptibility genes BRCA1 / BRCA2. Background technique [0002] The close relationship between BRCA1, BRCA2 susceptibility genes and breast cancer has received more and more attention, and the research is also deepening. The high correlation between BRCA1 and BRCA2 and familial hereditary breast cancer is beyond doubt. The detection of BRCA1 can reflect the occurrence and development of breast cancer, and can also screen out high-risk groups of breast cancer, ovarian cancer and other related malignant tumors, which is conducive to the early diagnosis and treatment of such diseases. Although the specific mechanism and pathway of BRCA2's tumor suppressor effect are not yet clear, breast cancer and several other malignant tumors, such as prostate cancer and ovarian cancer, can be detected early by ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113
Inventor 陈伟
Owner 杭州美中疾病基因研究院有限公司
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