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Plant salt tolerance-related protein IbERD3 as well as encoding gene and application of plant salt tolerance-related protein IbERD3

A plant and gene technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve problems affecting food production and restrictions, achieve broad application space and market prospects, increase salt tolerance, and increase plant height.

Active Publication Date: 2014-08-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of large areas of salinized land has seriously affected food production and has become the main factor restricting agricultural production

Method used

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  • Plant salt tolerance-related protein IbERD3 as well as encoding gene and application of plant salt tolerance-related protein IbERD3
  • Plant salt tolerance-related protein IbERD3 as well as encoding gene and application of plant salt tolerance-related protein IbERD3
  • Plant salt tolerance-related protein IbERD3 as well as encoding gene and application of plant salt tolerance-related protein IbERD3

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the acquisition of IbERD3 protein and its coding gene

[0027] Sweet potato salt-tolerant mutant LM79 (LM79 for short): References to this biological material: He Shaozhen. In vitro screening of sweet potato salt-tolerant mutants and cloning of salt-tolerant candidate genes. Doctoral dissertation of China Agricultural University, 2008.

[0028] 1. Extraction and purification of total RNA from leaves

[0029] About 2 g of expanded leaves of LM79 sterile seedlings were taken, ground into powder in liquid nitrogen, added to a 10 mL centrifuge tube, and total RNA was extracted with the Applygen Plant RNA Extraction Kit (Applygen Technologies Inc, Beijing). mRNA was purified from total RNA using the QIAGEN Oligotex Mini mRNA Kit (QIAGEN, GmbH, Germany). Take 1 μL of mRNA for 1.2% agarose gel electrophoresis to detect its integrity, and take another 2 μL of mRNA to dilute to 500 μL, and use a UV spectrophotometer to detect its quality (OD 260 ) and purity (OD ...

Embodiment 2

[0056] Embodiment 2, the application of IbERD3 protein in improving plant salt tolerance

[0057] 1. Construction of recombinant plasmid pCBIbERD3

[0058] 1. Synthesize the double-stranded DNA molecule shown in sequence 2 of the sequence listing.

[0059] 2. Using the double-stranded DNA molecule synthesized in step 1 as a template, a primer pair composed of F1 and R1 is used for PCR amplification to obtain a PCR amplification product.

[0060] F1: 5'- AGATCT ATGGCGATTTCGG-3' (underline marks the recognition sequence of Bgl II digestion);

[0061] R1: 5'- CACGTG TTATGAACTGGCATTT-3' (underlined Pml I enzyme recognition sequence).

[0062] 3. The PCR amplification product obtained in step 2 was double digested with restriction endonucleases Bgl II and Pml I, and the digested product was recovered.

[0063] 4. Digest the pCAMBIA3301 vector with restriction endonucleases Bgl II and Pml I to recover a vector backbone of about 9284bp.

[0064] 5. Ligate the digested product...

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Abstract

The invention discloses a plant salt tolerance-related protein IbERD3 as well as an encoding gene and an application of the plant salt tolerance-related protein IbERD3. The protein provided by the invention is from a sweet potato (Ipomoea batatas), is named as IbERD3 and is a protein as shown in (a) or (b), wherein (a) is a protein composed of an amino acid sequence as shown in a sequence 1 in a sequence table, and (b) is a protein formed by substituting and / or deleting and / or adding one or more amino acid residues in the amino acid sequence as shown in the sequence 1, is related to the plant salt tolerance and is derived from the sequence 1. The IbERD3 gene is induced to the sweet potato to obtain an IbERD3 transgenic sweet potato which is remarkably improved in salt tolerance as comparison with a wild sweet potato. The protein IbERD3 and the encoding gene thereof provided by the invention have important application values in improving the salt tolerance of plants. The plant salt tolerance-related protein IbERD3 is wide in application space and market prospect in the agricultural field.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant salt tolerance related protein IbERD3 and its coding gene and application. Background technique [0002] There are large areas of salinized land in the world. According to statistics, there are 3.8×10 8 hm 2 In saline-alkali land, there is also secondary salinized land accounting for 33% of the cultivated land in irrigated areas. Soil salinization seriously affects the development of modern agriculture. As far as our country is concerned, nearly one-tenth of the 1.8 billion mu of cultivated land in the country is secondary salinized land, and there are also 2×10 7 hm 2 Saline wasteland. Generally speaking, a salt concentration of 0.2% to 0.5% will affect the growth of crops, but the salinity in saline-alkali land is mostly 0.6% to 10%. The existence of a large area of ​​salinized land has seriously affected food production and has become the main factor restricting agri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N1/21A01H5/00
CPCC07K14/415C12N15/8273
Inventor 刘庆昌翟红何绍贞刘德高
Owner CHINA AGRI UNIV
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