Extraction method for limonin

Abstract

The invention discloses an extraction method for limonin. The extraction method includes the following steps of (1) raw material extracting in a soaked mode or juicing, wherein a raw material is directly soaked in water or directly juiced through a squeezer to obtain an extraction solution; (2) metal salt adding and pH adjusting, wherein sulfate solid matter or a sulfate saturated solution is added to the extraction solution, and the pH is adjusted to be 3-7; (3) heating and temperature keeping, wherein the solution is heated to 20 DEG C-100 DEG C, and the temperature is kept for 10 min-110 min; (4) centrifuging, the solution is centrifuged for 10 min at the rotating speed of 2500*g to obtain a limonin precipitate. Compared with a traditional method, the raw material can be directly soaked to obtain the extraction solution or directly juiced to obtain a squeezed solution without being dried by adopting the method, most of the limonin in the water is recovered through a precipitation method, complex equipment is not needed, and the extraction method is simple and convenient to operate and low in technological cost.

Description

technical field [0001] The invention relates to a method for extracting limonin, in particular to a method for extracting limonin by precipitation. Background technique [0002] Limonoid analogues are a class of highly oxidized tetracyclic triterpenoids mainly found in Rutaceae and Meliaceae plants. Since Bemay (1841) isolated limonin for the first time, people have successively isolated different limonin analogues. At present, more than 300 limonin analogues have been isolated from citrus plants and their hybrid plants. [0003] In recent decades, it has been found that limonoid analogues in citrus fruits have many biological functions: antimalarial, antibacterial, anti-inflammatory, analgesic, and insecticidal functions, which can effectively prevent pathogenic bacteria from invading the animal body, and can expel Parasites in animals; it can stimulate the activity of the main detoxifying enzyme in the body - glutathione transferase, and has strong anticancer activity; a ...

AI Technical Summary

Problems solved by technology

Using traditional methods, such methods are costly and time-consuming, which is not conducive to the industrial production of limonin, and part of limonin exists in aqueous solution in the form of limonin A cyclic lactone soluble, so it is difficult to separate

Some researchers use resin to adsorb limonin in aqueous solution, and then use organic solvent to elute the adsorbed limonin. This method also needs to use a large amount of organic solvent, and the organic solvent needs to be recovered, and the process cost is high.