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Method for extracting common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating common traditional Chinese medicine through MALDI-TOF-MS

A technology of traditional Chinese medicinal materials and traditional Chinese medicine, which is applied in the field of licorice, ginseng, and astragalus, can solve the problems of less application of identification of traditional Chinese medicinal materials, and achieve the effects of easy promotion and standardization, strong method versatility, and short analysis time

Inactive Publication Date: 2014-08-27
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

[0005] At present, MALDI-TOF-MS has been successfully applied in the identification of microorganisms, but there are relatively few applications in the identification of Chinese medicinal materials.

Method used

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  • Method for extracting common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating common traditional Chinese medicine through MALDI-TOF-MS
  • Method for extracting common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating common traditional Chinese medicine through MALDI-TOF-MS
  • Method for extracting common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating common traditional Chinese medicine through MALDI-TOF-MS

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Experimental program
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Effect test

Embodiment 1

[0022] instrument:

[0023] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (ultrafle Xtreme MALDI-TOF-MS), Brücke, Germany ；

[0024] YS-04 small high-speed pulverizer, Beijing Yanshan Zhengde Machinery Equipment Co., Ltd.;

[0025] 70mm (MD77) dialysis bag, Cutoff is 3KDa, USA;

[0026] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory;

[0027] KFLOW pure water machine, KFLOW company;

[0028] ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory;

[0029] Adjustable pipette, Thermo Labsystems;

[0030] Vortex mixer, Shanghai Huxi Instrument Analysis Factory.

[0031] 2. Reagents: Tris base, Sigma-Aldrich; other reagents are analytical reagents.

[0032] 3. Steps, taking the traditional Chinese medicine Astragalus as an example

[0033] 1. Chinese herbal medicine sample processing and protein extraction

[0034] Take 15g of sliced ​​dried Astragalus membranaceus, and pulverize throu...

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Abstract

The invention discloses a method for extracting a common traditional Chinese medicine from traditional Chinese medicinal materials and authenticating the common traditional Chinese medicine through MALDI-TOF-MS, and belongs to the technical field of biology. According to the method, the traditional Chinese medicinal materials are smashed at high speed, total protein and polypeptide are extracted from the traditional Chinese medicinal materials, dialysis desalination is conducted, then the MALDI-TOF-MS is adopted for detection, and a traditional Chinese medicinal material protein map is obtained. The simple protein extracting technology and Malditof are combined for authenticating the protein maps of the common traditional Chinese medicinal materials such as astragalus membranaceus, ginseng and liquorice. The method has the advantages of being easy and reliable to implement, and provides selections and references for authenticating the common traditional Chinese medicinal materials.

Description

technical field [0001] The invention relates to a method for extracting common Chinese medicines from Chinese medicinal materials and identifying them by MALDI-TOF-MS, in particular to a method for identifying commonly used Chinese medicinal materials astragalus, ginseng and licorice by using Malditof, and belongs to the field of biotechnology. Background technique [0002] Chinese herbal medicines are the raw materials of Chinese herbal decoction pieces, Chinese herbal extracts and Chinese patent medicines. Its front end is medicinal plants, medicinal animals, medicinal minerals and their specific medicinal parts, or the base of Chinese herbal medicines. Accurate identification of Chinese medicinal materials is a basic link in the operation and development of Chinese medicine, and has always attracted attention. The relevant national medicinal material standards mainly confirm the source species based on taxonomic research, limit the medicinal parts, and basically clarify t...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K36/481A61K36/484A61K38/00G01N30/72
Inventor 马伟胥传来王文彬匡华徐丽广刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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