Method for removing lipoprotein and lipid in goose/duck yolk by using freezing method

Abstract

The invention discloses a method for removing lipoprotein and lipid in goose / duck yolk by using a freezing method. The method comprises the following steps of (1) cleaning and disinfecting a fresh egg laid by a goose / duck supplementarily immunized by using a goose vaccine; (2) separating egg white from yolk; (3) extracting 1 part of separated yolk according to a certain volume, adding 3 times of distilled water with the same volume to dilute, uniformly stirring, and storing under freezing at the temperature of 18 DEG C below zero for 24h and longer to ensure that the yolk is solidly frozen; (4) taking out the solidly frozen yolk, unfreezing, and filtering to obtain colorless and transparent filtrate capable of purifying immunoglobulins IgY. The goose vaccine in the step (1) is a gosling plague vaccine, a goose paramyxovirus vaccine or an avian influenza vaccine. By using a physical freezing separation method, a yolk sediment is sufficiently utilized, and no chemical organic solvents are adopted, so that the cost can be remarkably reduced.

Description

technical field [0001] The invention relates to a method for removing lipoprotein and lipid in goose and duck egg yolk by freezing method. Background technique [0002] Antibody (immunoglobulin, Ig) refers to a type of antibody that is synthesized and secreted by plasma cells and can specifically recognize and bind to corresponding antigens after the body is stimulated by foreign antigenic substances. globulin. The geese and ducks immunized with specific antigens can produce corresponding specific antibodies. Studies have confirmed that the yolk of poultry has a concentration effect on antibodies, making them continuously stored in the yolk. This antibody is called yolk antibody (Immunoglobulin of yolk) , IgY), egg yolk antibody is a major discovery in the field of biological science research today. [0003] Separation, extraction and purification of IgY: The separation is mainly to remove the high content of fat and lipoprotein in egg yolk to obtain the water-soluble frac...

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Problems solved by technology

[0006] 1) Organic matter precipitation method, agent solvent precipitation method, amino acid salt or sodium sulfate method, polyvinyl alcohol (PEG) method, octanoic acid method, water dilution method (WD method), supercritical gas extraction method (SFE method), The biggest disadvantage of these methods is the consumption of a large amount of organic matter and organic solvents;

[0007] 2) Water dilution method, the dilution factor is large, the separation effect is not ideal, and the supercritical gas extraction method requires large equipment investment and high cost;

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