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Method of detecting yield of royal jelly by LOC409360 gene of bee

A technology for genetic testing and royal jelly, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of small bee species and sample size, and sample selection is not very scientific, and achieve good yield performance.

Inactive Publication Date: 2014-07-30
ZHEJIANG UNIV
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Problems solved by technology

They believed that the number of bee species and sample size in the above experiment was too small, and the sample selection was not very scientific. It cannot be simply inferred that W316bp can be used as a marker of high-yielding royal jelly just because three bees with high-yielding royal jelly have it but not among the low-yielding bees. Potentially related to other biological traits of four different bee species

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  • Method of detecting yield of royal jelly by LOC409360 gene of bee

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Embodiment 1

[0038] A method for detecting royal jelly output with honeybee LOC409360 gene, comprising the following steps:

[0039] 1. Bee sample collection

[0040] ① During the jelly production season, 30 colonies of bees in the Qiandao Lake Experimental Bee Farm in Zhejiang Province were used to produce royal jelly for 5 consecutive times, and the weight of royal jelly and the amount of royal jelly received by each colony were weighed and recorded.

[0041] ②5 colonies with high royal jelly production, 5 colonies with low royal jelly production, and 4 other bee colonies whose royal jelly production was between high and low yields with a gradient trend were screened out, and a total of 14 bee colonies were selected as the investigated bee colonies. The royal jelly production range of the royal jelly high-yield bee colony and the royal jelly low-yield bee colony is that the high-yield colony produces no less than 100 grams of royal jelly per three-day colony, and the low-yield colony pro...

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Abstract

The invention relates to a method of detecting yield of royal jelly by LOC409360 gene of a bee. The method comprises the following steps: bee sample collection; extraction of RNA (Ribonucleic Acid) at the head of the bee; synthesis of cDNA (Complementary Deoxyribonucleic Acid) at the head of the bee; primer designing; qRT-PCR (Quantitative Real-Time Polymerase Chain Reaction) reaction system and condition; and yield capability identification of royal jelly. In the level of molecular biology, the invention provides a fluorescent quantitative PCR method which can more scientifically and accurately identify the yield capability of a swarm in order to solve the problem that production of royal jelly is hugely affected by conditions such as trend of the swarm, nectar sources, environment and climate, thereby greatly shortening the inspection period of production trait of royal jelly, accelerating the breeding speed of bees, reducing the inspection cost and breeding cost of production trait of royal jelly and further alleviating the working strength of inspection of yield of royal jelly in the field.

Description

technical field [0001] The invention relates to a method for identifying the yield performance of royal jelly by using fluorescent quantitative PCR technology, in particular to a method for detecting the yield of royal jelly with honeybee LOC409360 gene. Background technique [0002] my country is a big beekeeping country, and it is also the main producer of royal jelly. More than 90% of the world's royal jelly is produced in China, and royal jelly-yielding bees are a unique bee resource in my country. For a long time, my country's bee industry scientific and technological workers have been committed to the related research on the genetic breeding of bees with high royal jelly yield, in order to find the genetic markers related to the high-yield traits of royal jelly, and serve the high-yield breeding of bee royal jelly. The genetic markers related to the high yield of royal jelly were studied at the level of morphology, cytology, biochemistry and molecular level. An overvi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q1/6851C12Q2600/124C12Q2600/158C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 苏松坤潘娇陈盛禄
Owner ZHEJIANG UNIV
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