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A kind of anaerobic ammonia oxidation bacteria immobilized bioactive filler, preparation and application

A technology of anaerobic ammonium oxidation and bioactivity, which is applied in the field of fixed bioactive fillers, can solve the problems of unsatisfactory bioactive fillers, poor stability of bioactive fillers, and easy detachment of embedded bodies, etc., and achieve the effect of substrate mass transfer Good, strong acid and alkali resistance, strong water impact resistance

Active Publication Date: 2016-05-25
BEIJING UNIV OF TECH
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Problems solved by technology

After experimental research and comparison, we found that the bioactive fillers prepared based on the traditional filler structure and embedding method did not perform well in the test, and there were many problems such as poor overall stability of the bioactive filler, easy falling off of the embedded body, and water-soluble swelling. question

Method used

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  • A kind of anaerobic ammonia oxidation bacteria immobilized bioactive filler, preparation and application

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Embodiment Construction

[0028] The present invention will be further specifically described below in conjunction with implementation cases, but the implementation of the present invention is not limited to this.

[0029] 1. Treatment of anammox bacteria liquid

[0030] Centrifugally concentrate the anaerobic ammonia oxidizing bacterial suspension after the enrichment culture, and the bacterial concentration is 3.0×10 9 A / mL anammox bacteria concentrate, take out 3L of anammox bacteria concentrate for use.

[0031] 2. Treatment of anammox bacteria liquid

[0032] Take 1.0kg of polyvinyl alcohol water bath and heat it to 90°C and dissolve it in 7L of water to obtain a polyvinyl alcohol solution, cool to 30°C and fully mix with the anammox bacteria concentrate obtained in step 1, add water to make the volume up to 10L to get embedded The concentration of polyvinyl alcohol in the embedding liquid is 100g / L.

[0033] 3. Preparation of Embedding Vector

[0034] Stack four layers of polyvinyl alcohol non-woven fabric...

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Abstract

The invention discloses anaerobic ammonia oxidizing bacteria fixed-type bioactive fillers, and preparation and an application thereof, and belongs to the technical field of water treatment. Fillers are each composed of a carrier and an embedding body and are fixed on a prefabricated frame to form the fixed-type bioactive fillers; each carrier is prepared by sewing multiple layers of polyvinyl alcohol non-woven fabrics and one layer of textile fabric; the embedding bodies are formed by boric acid cross-linking immobilization of an embedding liquid; the embedding liquid is formed by mixing an anaerobic ammonia oxidizing bacteria suspension and a polyvinyl alcohol solution. Each carrier is soaked in the embedding liquid, through boric acid secondary cross-linking, the non-woven fabrics and the bacteria embedding body are firmly bound together, the embedding body penetrates through textile fabric meshes to form a riveting fixing structure and does not easily fall off, and the good tensile property of the textile fabric improves the overall stability of the bioactive filler. The prepared bioactive fillers not only solve the problems that anaerobic ammonia oxidizing bacteria are low in concentration, are easy to lose and the like, but also have the advantages of strong strength, good stability, strong water flow impact resistant ability and the like, shorten the reaction start-up time, and have broad application prospects.

Description

Technical field [0001] The invention relates to the preparation and application of a fixed biologically active filler of anammox bacteria in the field of water treatment, in particular to a fixed biologically active filler using textiles and polyvinyl alcohol non-woven fabrics as carriers. Background technique [0002] Anaerobic ammonia oxidation technology (ANAMMOX) is currently the most economical biological denitrification pathway known. A study by Strous et al. of Delft University in the Netherlands showed that the biological reaction process including cell synthesis in the anaerobic ammonia oxidation process can be expressed as the formula ( 1) [0003] NH 4 + +1.32NO 2 ‐ +0.066HCO 3 ‐ +0.13H + → [0004] 1.02N 2 +0.26NO 3 ‐ +0.066CH 2 O 0.5 N 0.5 +2.03H 2 O (1) [0005] It can be seen from the biological reaction characteristics of ANAMMOX that compared with traditional nitrification and denitrification technology, ANAMMOX has the advantages of low oxygen demand, low operating ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08
Inventor 杨宏王猛管清坤陈伟鄢琳王小乐吴城锋孟婷陶慕翔尚海源胡希佳赵月兰王玉洁姚仁达
Owner BEIJING UNIV OF TECH
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