Method for synthesizing isomalto oligosaccharides through fully enzymatic transformation path
A kind of isomaltooligosaccharide and enzymatic technology, which is applied in the field of synthesizing isomaltose oligosaccharide through the whole enzymatic conversion route, can solve the problems of inconvenient control of process parameters, difficulty in realizing continuous production, unstable source, etc., and achieve the reduction of ion exchange burden, save the amount of acid and alkali, and stabilize the reaction process
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[0022] Reagents used in this example: activated carbon for sugar: Dali activated carbon factory in Daxing County, Beijing; styrene-based macroporous strong acid cation resin, styrene-based macroporous weakly alkaline anion resin: food grade, Blower Resin Company. Glucose, analytically pure; maltose, isomaltose, maltotriose, panose, isomaltose, maltotetraose, isomalttetraose standard products, all reagents from Sigma; Fehlin A solution, Fehlin B solution, acetic acid Zinc, glacial acetic acid, potassium ferrocyanide, hydrochloric acid; Acetonitrile, chromatographically pure; High-temperature α-amylase was purchased from Novozymes; Compound glucoamylase was purchased from Genenco Biotechnology Co., Ltd.;
[0023] The instrument used in this example: Mettler Toledo AL204 analytical balance (METTLER TOLEDO Instruments Co., Ltd., Switzerland); burette; DK-S12 electric heating constant temperature water bath (Shanghai Senxin Experimental Instrument Co., Ltd.); DZG-6050 vacuum Drying bo...
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