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Bacterial strain for degrading dye with high efficiency

A high-efficiency, strain-based technology, applied in bacteria, microbial-based methods, water/sludge/sewage treatment, etc., to achieve good ecological efficiency and application prospects, avoid environmental pollution, and broad-spectrum dye degradation capabilities

Active Publication Date: 2014-07-23
厦门众仁合美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no research literature and patent reports on the use of Bacillus lysinus for dye decolorization in China.

Method used

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  • Bacterial strain for degrading dye with high efficiency
  • Bacterial strain for degrading dye with high efficiency
  • Bacterial strain for degrading dye with high efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Lysinibacillus sp . FS1 Isolation of strains

[0045] 1. Strain activation and enrichment

[0046] Take 50mL of activated sludge sample (taken from the wastewater treatment aeration tank of Guangzhou Industrial Park, Guangdong Province), put it into an Erlenmeyer flask containing 50mL of sterile water (with several glass beads inside), and shake it on a shaker for 30 minutes. Loose mud. Take 5mL of the dispersed mud sample, inoculate it into 100mL of enriched medium, add 30mg / L of Acid Red B dye, and culture with shaking at 37°C and 200rpm for 16-24 hours.

[0047] The composition of the enrichment medium is: beef extract 1g, peptone 2g, glucose 3g, K 2 HPO 4 2g, NaH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·7H 2 O 0.02g, FeCl 3 ·7H 2 O 0.01g, CaCl 2 0.02g, 1L sterile water, pH 7.2; sterilize the culture medium at 121°C for 15-30 minutes.

[0048] 2. Strain screening and purification

[0049] Dilute the enriched culture solution with obvious decolorization effec...

Embodiment 2

[0051] Example 2 Lysinibacillus sp. Test on the decolorization effect of FS1 on different concentrations of methylene blue

[0052] The strain FS1 that efficiently degrades the dye of the present invention is inoculated into a liquid medium, and cultured on a shaker at 30-37°C and 150 rpm for 10-24 hours to obtain the culture broth of the strain; the composition of the liquid medium is: 2g glucose, 1g ammonium chloride, NaH 2 PO 4 0.5g, K 2 HPO 4 2g, FeSO 4 ·7H 2 O 0.01g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 0.02g, 1L water, pH7.2.

[0053] Take the logarithmic growth period (OD 600 =1) Lysinibacillus sp. FS1 culture medium, 1% (V / V) into 100mL of decolorizing basic medium, adding different concentration gradients (80mg / L, 500mg / L, 2000mg / L, 3000mg / L, 4000mg / L, 5000mg / L) Methylene blue dye. The three were cultured in parallel at room temperature, and the absorbance of the culture medium was measured every 5-24 h.

[0054] The decolorization rate was used to investigate the decoloriza...

Embodiment 3

[0058] Example 3 Lysinibacillus sp. The decolorization effect test of FS1 on different concentrations of neutral red

[0059] Referring to Example 2, the strain was cultured, and the logarithmic growth phase (OD 600 =1) strain FS1 culture solution, 1% (V / V) into 100mL decolorizing basic medium, adding different concentration gradients (80mg / L, 300mg / L, 500mg / L, 1000mg / L, 2000mg / L) neutral red dye. Others are the same as in Example 2.

[0060] The test results are attached Figure 4 As shown, when the concentration of neutral red is 300~1000mg / L, the decolorization rate basically reaches about 90% of the maximum decolorization rate within 10h. When the concentration increases, the strain decolorization time increases by about 48h. As the concentration of neutral red increases, the ability of the strain to decolorize it gradually decreases, from 99% to 30%. Strain Lysinibacillus sp. FS1 The highest decolorization concentration of neutral red is as high as 2000mg / L.

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Abstract

The invention discloses a bacterial strain for degrading dye with high efficiency. The bacterial strain is Lysinibacillussp ((i) Lysinibacillussp.( / i))FS1, and is preserved in the China Center for Type Culture Collection with the preservation number is CCTCC NO: M2013561 on Nov, 10th, 2013. The bacterial strain has the advantage of rapid growth and strong environment adaptability, has dye degradation capability with wide spectrum, and has good decolouring effect to a plurality of dyes, maximum decolourization percentage can be reached by culturing for 10 hours, the percent of decolourization can reach more than 90%, and the maximum decolouring concentration can reach 5000mg / L. The bacterial strain can be used for decolouring the printing and dyeing waste water and correlated dye waste water, can effectively avoid the dye molecules which are difficulty performed with natural degradation to directly enter in the water body for environment pollution, no secondary pollution is generated, and the bacterial strain has good ecology efficiency and application prospect. The Lysinibacillussp can be better used for degrading the dye, fills the blank of domestic technology, and provides a powerful technical base for solving the dye sewage treatment, especially the decolouring problem.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a bacterial strain for efficiently degrading dyes. Background technique [0002] Synthetic dyes are widely used in printing and dyeing, leather, food, cosmetics, paper products and other industries, resulting in a large amount of dye wastewater. Because dye wastewater has the characteristics of high chroma, complex components, strong chemical stability, and poor biodegradability, a large amount of dyes enter the environment with the discharge of industrial wastewater, and most azo dyes have potential triple effects (teratogenicity) , carcinogenic, mutagenic), and have serious harm to the environment and human health. At present, the annual consumption of dyes in the Pearl River Delta region of my country exceeds 2×10 4 tons, of which 10-20% of the dyes are directly discharged into the environment, and azo dyes account for about 50% of the total discharge. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 陶然杨扬苏萌赵建成钟胜强
Owner 厦门众仁合美生物科技有限公司
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