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Systems and methods for detecting allergens

A technology for allergens and food, applied in the field of systems and methods for detecting allergens, which can solve problems such as expensive, lack of specificity, and single allergens

Inactive Publication Date: 2016-12-21
DH TECH DEVMENT PTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional methods for detecting and / or quantifying allergens in food samples typically employ immunoassays, which can have a number of disadvantages
For example, one such method called enzyme-linked immunosorbent assay (ELISA) requires the use of expensive kits and is at best semi-quantitative
Additionally, conventional immunoassays are generally limited to a single allergen per screen and frequently result in false positives due to lack of specificity

Method used

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  • Systems and methods for detecting allergens
  • Systems and methods for detecting allergens
  • Systems and methods for detecting allergens

Examples

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Embodiment Construction

[0058] Those skilled in the art will appreciate that the methods and kits described herein are non-limiting exemplary embodiments and that the scope of Applicants' disclosure is defined only by the claims. While applicants' teachings have been described in connection with various embodiments, it is not intended that applicants' teachings be limited to such embodiments. On the contrary, applicant's teachings cover various alternatives, modifications, and equivalents, as would be understood by those skilled in the art. Features illustrated or described in connection with one exemplary embodiment may be used in combination with features of other embodiments. Such modifications and variations are intended to be included within the scope of applicant's disclosure.

[0059] According to various embodiments, methods are provided for using mass spectrometry to screen a sample for the presence or amount of the following proteins by detecting amino acid sequences characteristic of one ...

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PUM

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Abstract

Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.

Description

technical field [0001] The present invention generally relates to kits and methods for the detection and quantification of allergens in samples, such as food samples, using mass spectrometry. Background technique [0002] Food allergies can induce deleterious immune responses such as dermatitis, asthma, gastrointestinal injury, anaphylactic shock, etc. Conventional methods for detecting and / or quantifying allergens in food samples typically employ immunoassays, which can have a number of disadvantages. For example, one such method called enzyme-linked immunosorbent assay (ELISA) requires the use of expensive kits and is at best semi-quantitative. In addition, conventional immunoassays are generally limited to a single allergen per screen and frequently result in false positives due to lack of specificity. [0003] Accordingly, there is a need in the art for improved kits and methods for the detection and quantification of allergens in food samples. Contents of the invent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N30/72C12Q1/37
CPCG01N30/7233G01N33/6848G01N2030/8831G01N2333/415G01N2333/4713G01N2333/4731G01N2333/77G01N2333/976G01N2800/24
Inventor 斯蒂芬·J·洛克萨巴辛·普卡雅斯特哈布莱恩·L·威廉姆森董克玲
Owner DH TECH DEVMENT PTE
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