Fluorescence quantitative automatic detection device for immunoassay microfluidic chip

Abstract

The invention discloses an automatic immunodetection micro-fluidic chip fluorescent quantification detection device which comprises an excitation light source, an exciting light focusing module, a fluorescence collection module, a photoelectric conversion module, a linear motion platform module, a circuit module, an automatic reaction execution module and a waste reaction liquid removal module. In the detection process, a chip is subjected to one-dimensional quantitative scanning detection through linear light spots obtained by the exciting light focusing module; a controller obtains the concentration or cell number of a sample to be detected based on fluorescence signal intensity distribution information in a chip two-dimensional rule detection area; the detection result is connected with a mobile phone through Bluetooth and is transmitted to medical institutions in real time, and remote diagnosis or epidemic disease distribution area monitoring is realized. A linear light spot one-dimensional scanning detection method is adopted, and quantification of micro-fluidic chip detection is realized. Moreover, by means of the automatic reaction execution module, automation of the micro-fluidic chip detection is realized; due to the integrated waste reaction liquid removal module, the allowed reaction sample amount is increased, and the detection sensitivity is improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a fluorescent quantitative automatic detection device for an immune detection microfluidic chip. Background technique [0002] The immunoassay method is an analytical method that uses the specific binding reaction of antigens and antibodies to detect various targets (drugs, hormones, proteins, microorganisms, etc.). The microfluidic chip rapid detection method and device based on the principle of immunoassay has obvious advantages in the field of rapid diagnosis or bedside diagnosis due to its low cost, convenience, and simple operation, and is becoming the next generation of development in the world. Medical diagnostic platform. The fluorescence detection method has the characteristics of high sensitivity and wide dynamic range, which can effectively improve the comprehensive performance of microfluidic chip immunoassay. [0003] Existing fluorescent quantitative detectors ...

AI Technical Summary

Problems solved by technology

The two-dimensional fluorescence scanning detection method reported in the literature can obtain a complete fluorescence signal intensity distribution by performing point-by-point two-dimensional scanning on the detection area. However, the point scanning detection method has complex detection systems, cumbersome operations, and long detection time. , and the cost is relatively high, etc.

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