Cross pollination method for dendrobium officinale

Abstract

The invention relates to a simple and efficient cross pollination method for dendrobium officinale. The method comprises the following steps: (1) collecting pollen; (2) preparing dendrobium officinale flower juice; (3) preparing a pollen suspension; (4) pollinating; and (5) performing management at the later stage of fruit setting. A dendrobium officinale pollen load suspension is obtained by performing ultrasonic homogenization on pollen grains and the dendrobium officinale flower juice, and dendrobium officinale flowers are pollinated by the dendrobium officinale pollen load suspension by using a rubber-head burette, so that damages to stigmas of female parents are avoided, the fruit setting rate is high (over 98.2 percent), inane fruits are prevented, mature dendrobium officinale capsules can be obtained by sitting for 120 days, and fruits are uniform and plump. The method has the characteristics of easiness, convenience, high efficiency and high operability, the pollinating efficiency is 5-10 times that of a conventional pollinating method, the pollinating efficiency of dendrobium officinale is increased greatly, the application value is extremely high, and a novel high-speed and effective way is provided for hand pollination of dendrobium officinale.

Description

technical field [0001] The invention relates to a hybrid breeding method of rare medicinal plants, in particular to a hybrid pollination method of dendrobium officinale. Background technique [0002] Dendrobium of ficinale (Demdrobium of ficinale) is a perennial herbaceous plant of the genus Dendrobium orchidaceae. It is a traditional precious and rare Chinese medicinal material. At present, Dendrobium officinale has been listed as a national key protected medicinal plant and a second-class protected plant in the world. [0003] Due to the special physiological structure of Dendrobium officinale flowers, the fruiting rate of Dendrobium officinale is extremely low under natural conditions. Generally, Dendrobium officinale is in full bloom in May, and the flowering period is 10-15 days. Its fruit is a capsule, which is easy to crack when mature, and the seeds float with the wind. Scattered, the seed coat of these tiny seeds only contains immature embryos, without endosperm, s...

AI Technical Summary

Problems solved by technology

The acquisition of Dendrobium seed embryos has become one of the factors for the industrialization of Dendrobium officinale. At present, Dendrobium seed embryos are mainly obtained through traditional artificial pollination. Disadvantages such as low production efficiency, low fruit setting rate and high empty fruit rate

As disclosed on September 23, 2009, the Chinese invention patent application "Method for Hybrid Pollination of Dendrobium officinale" with the application number 200910010107.3 provides a hybridization technique of Dendrobium candidum, but it needs to manually remove the pollen grains of the male parent with tweezers Send it to the stigma nest of the female parent, which will easily cause physical damage to the stigma during operation, and the fruit setting rate of this method should not exceed 80.6%.