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Method of test strip detecting concentration of analyte of sample, and test strip

A diffusion factor and test piece technology, applied in biochemical equipment and methods, measuring devices, microbial determination/inspection, etc.

Active Publication Date: 2014-06-25
TYSON BIORESEARCH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In summary, for users, none of the methods disclosed in the prior art is a good choice

Method used

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  • Method of test strip detecting concentration of analyte of sample, and test strip
  • Method of test strip detecting concentration of analyte of sample, and test strip
  • Method of test strip detecting concentration of analyte of sample, and test strip

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Embodiment Construction

[0036] The present invention will be illustrated by the following examples, wherein those skilled in the field of the present invention should understand that the following examples are only used to illustrate the present invention and do not limit the scope of the present invention.

[0037] Please refer to figure 1 , figure 1 It is a schematic exploded view illustrating a test piece 100 according to the first embodiment of the present invention. Such as figure 1 As shown, the test piece 100 can include a substrate 110, an electrode layer 120, an insulating layer 130, a reagent layer 140, a spacer 150, a first covering layer 160, and a second covering layer 170, wherein the substrate 110 It can be made of plastic material (for example, polyethylene terephthalates (PET), vinyl polymers, polyimides or polyesters).

[0038] The substrate 110 supports an electrode layer 120 , wherein the electrode layer 120 has a working electrode 121 , an auxiliary electrode 122 and a referen...

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Abstract

The invention discloses a method of a test strip detecting concentration of an analyte of a sample, a test strip with three electrodes, and a method of testing medium diffusion factors in the analyte of the sample by means of the test strip. The method of the test strip detecting concentration of the analyte of the sample includes placing the sample in a reaction region of the test strip, wherein the analyte reacts with an enzyme to generate a plurality of electrons, and the plurality of electrons are transferred to a working electrode of the reaction region through a mediator; applying an electrical signal to the working electrode; measuring a first current through the working electrode during a first period; the mediator generating an intermediate according to the electrical signal during a second period; measuring a second current through the working electrode during a third period; calculating initial concentration of the analyte according to the first current; calculating a diffusion factor of the intermediate in the sample according to the second current; and correcting the initial concentration to generate new concentration of the analyte according to the diffusion factor.

Description

technical field [0001] The invention relates to a method for detecting the concentration of analyte in a sample by a test piece, a three-electrode test piece and a method for detecting the medium diffusion factor in a sample by using the test piece, especially a method using different electrodes in different periods of time. The invention relates to a method for detecting the concentration of the analyte in the sample and the medium diffusion factor in the sample by the electrical signal, and a test piece for detecting the concentration of the analyte in the sample and the medium diffusion factor in the sample. Background technique [0002] Electrochemical biosensors have been widely used to determine the concentration of different analytes (such as glucose, uric acid and cholesterol in biological fluids) in test samples. For example, in biological sample testing, a test strip can be inserted into a blood glucose meter, and a bodily fluid sample is dripped onto the test stri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327
CPCG01N27/3274C12Q1/001G01N27/3272G01N27/416
Inventor 李政哲陈文煌蔡瀚庆杨振宇
Owner TYSON BIORESEARCH INC
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