Proliferation culture medium for tissue culture of Jietu vaccinium vitisidaea
A proliferation medium and tissue culture technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems affecting the rapid development and slow growth of lingonberry
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Embodiment 1
[0034] Inoculate Jietu lingonberry into the improved WPM proliferation medium, and its medium components and contents include:
[0035] Macroelements: 1071.6 mg·L -1 Potassium nitrate, 370 mg·L -1 Magnesium sulfate, 96 mg·L -1 Calcium chloride, 170mg potassium dihydrogen phosphate;
[0036] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg sodium molybdate, 6.2 mg·L -1 boric acid;
[0037] Iron salt: 37.3 mg·L -1 Disodium edetate, 27.8 mg·L -1 ferrous sulfate;
[0038] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 Glycine;
[0039] Sucrose 30 g·L -1 , agar 8 g·L -1 ;
[0040] Zeatin 0.5 mg·L -1 ;
[0041] The rest is distilled water with a pH of 5.0;
[0042] The preparation method is as follows:
[0043] (1) Preparation of modified WPM medium:
[0044] (11) Preparation of 50-fold macroelement mothe...
Embodiment 2
[0058] Inoculate Jietu lingonberry into the improved WPM proliferation medium, and its medium components and contents include:
[0059] Macroelements: 1071.6 mg·L -1 Potassium nitrate, 370 mg·L -1 Magnesium sulfate, 96 mg·L -1 Calcium chloride, 170mg potassium dihydrogen phosphate;
[0060] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg sodium molybdate, 6.2 mg·L -1 boric acid;
[0061] Iron salt: 37.3 mg·L -1 Disodium edetate, 27.8 mg·L -1 ferrous sulfate;
[0062] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 Glycine;
[0063] Sucrose 30 g·L -1 , agar 9 g·L -1 ;
[0064] Zeatin 0.6 mg·L -1 ;
[0065] The rest is distilled water with a pH of 4.8;
[0066] The preparation method is as follows:
[0067] (1) Preparation of modified WPM medium:
[0068] (11) Preparation of 50-fold macroelement mothe...
Embodiment 3
[0081] The "Jietu" lingonberry was inoculated in the improved WPM proliferation medium, and its medium composition and content included:
[0082] Macroelements: 1071.6 mg·L -1 Potassium nitrate, 370 mg·L -1 Magnesium sulfate, 96 mg·L -1 Calcium chloride, 170mg potassium dihydrogen phosphate;
[0083] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg sodium molybdate, 6.2 mg·L -1 boric acid;
[0084] Iron salt: 37.3 mg·L -1 Disodium edetate, 27.8 mg·L -1 ferrous sulfate;
[0085] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 Glycine;
[0086] Sucrose 30 g·L -1 , agar 8 g·L -1 ;
[0087] Zeatin 0.8 mg·L -1 ;
[0088] The rest is distilled water with a pH of 4.9;
[0089] The preparation method is as follows:
[0090] (1) Preparation of modified WPM medium:
[0091] (11) Preparation of 50-fold macroele...
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