Construction method of doxorubicin nephropathy animal model

Abstract

The invention discloses a construction method of an Adriamycin nephropathy animal model, for solving the technical problem that an existing Adriamycin nephropathy animal model is complex in operation and low in success rate. The construction comprises the following steps: selecting rats according with experimental standards as a molding group; injecting Adriamycin to the rats of the molding group by caudal vein for the first time according to a using amount of 4mg / kg; one week later, injecting Adriamycin for the second time according to the using amount of 3.5mg / kg; from the first injection of Adriamycin to the rats in the molding group, feeding the rats with high protein feed for two weeks, and then feeding with a normal feed for 6 days; and from the first injection of the Adriamycin to the 20th day, reckoning the rats with the total amount of 24-hour urine protein being larger than 30mg into the Adriamycin nephropathy animal model. The construction method disclosed by the invention is simple, feasible, high in success rate, good in consistency and stability and capable of effectively reducing the animal death rate and is a relatively ideal animal model for further researching sertoli cytopathy.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of adriamycin nephropathy. Background technique [0002] Doxorubicin (ADR) is a quinone-containing anthracycline antibiotic, which can be metabolized and reduced to semiquinone free radicals in the kidney, which react with oxygen to generate reactive oxygen species, which induce lipid peroxidation in glomerular epithelial cells and affect glucose Protein metabolism destroys the structure and function of the filtration membrane, eventually leading to selective changes in the membrane filtration barrier and causing proteinuria. The nephrotoxic effect of doxorubicin and the stimulation of a large amount of proteinuria further induce glomerular resident cells and other inflammatory cells to produce and release various cytokines and inflammatory mediators, stimulate the proliferation of glomerular mesangial cells and Matrix ...

AI Technical Summary

Problems solved by technology

Because doxorubicin has strong toxic and side effects on the heart, liver, bone marrow, gastrointestinal system and other systems, a single high-dose injection makes the mortality rate of this model high, although the measures of shortening the modeling time and reducing the dosage of doxorubicin can Improve the survival rate of the model, but it will affect the modeling effect

Some people also use the method of right nephrectomy and repeated injection of ADR to make models, but in the experiment, rats will die due to surgical accidents or infection, and the death rate of rats will increase after injection of ADR

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