Homogeneous luminescent immunoassay method for simultaneous quantitative analysis of multiple components and kit used therefor

A quantitative analysis and homogeneous luminescence technology, which is applied in the field of clinical examination and diagnosis, can solve the problems of high detection cost, complex and unsuitable biochip technology, and achieve the effects of simple operation, saving specimen consumption, and high precision

Inactive Publication Date: 2016-01-13
TIANJIN NANKAI HOSPITAL
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Problems solved by technology

In addition, biochips are not suitable for the detection of routine clinical specimens due to their complex technology and high detection costs.

Method used

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  • Homogeneous luminescent immunoassay method for simultaneous quantitative analysis of multiple components and kit used therefor
  • Homogeneous luminescent immunoassay method for simultaneous quantitative analysis of multiple components and kit used therefor
  • Homogeneous luminescent immunoassay method for simultaneous quantitative analysis of multiple components and kit used therefor

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Embodiment Construction

[0026] Taking the simultaneous quantitative analysis of serum alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), sugar chain antigen 125 (CA-125), and ferritin (Ferr) as an example, the homogeneous luminescence immunoassay for simultaneous determination of multiple components is described in detail. Analytical methods (such as image 3 ): There are four receptor microspheres in the detection system, and the four receptor microspheres all contain the same chemiluminescence agent and different fluoresceins, among which 1# receptor microspheres contain FITC, and 2# receptor microspheres contain PE, 3# receptor microspheres contain EDC, 4# receptor microspheres contain PE-Cy5), each receptor microsphere is coated with a polyclonal antibody, 1# receptor microspheres are anti-AFP, 2 # acceptor microspheres are anti-CEA, 3# acceptor microspheres are anti-CA-125, and 4# acceptor microspheres are anti-Ferr. In addition, there are four biotinylated monoclonal antibodies in the sy...

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Abstract

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.

Description

technical field [0001] The invention relates to a homogeneous luminescent immunoassay method for simultaneous determination of multiple components, which is characterized in that multiple biomarkers in samples to be tested can be quantitatively analyzed at the same time, and can be applied to the field of clinical examination and diagnosis. Background technique [0002] Labeled immunoassay is an ultra-trace biological analysis method, which utilizes the high affinity between antigen-antibody and the high measurability of the label as a probe, and can accurately quantitatively analyze ultra-trace substances in biological samples. With the advantages of simple operation, high specificity and high sensitivity, it has become one of the important detection methods in the fields of biology and clinical medicine. [0003] Luminol oxygen pathway immunoassay (luminoloxygenchannelimmunoassay, LOCI) technology is a new type of marker immunoassay, similar to LOCI technology, Shanghai Bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533G01N21/64
CPCG01N21/6402G01N33/53G01N33/533
Inventor 常艳敏张盈莹朱黎娜侯丽英李会强吴嫱
Owner TIANJIN NANKAI HOSPITAL
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