Microfluidic microbial culture detection chip

A technology for microbial culture and detection chips, which is applied in the field of microfluidics and can solve the problems of lack of culture liquid detection structure and inability to measure concentration.

Active Publication Date: 2016-01-27
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chip can only determine the number of cells in the culture solution by cell counting, lacks a culture solution detection structure, and cannot measure the concentration of specific components in the culture solution

Method used

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  • Microfluidic microbial culture detection chip
  • Microfluidic microbial culture detection chip
  • Microfluidic microbial culture detection chip

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Embodiment Construction

[0030] The microfluidic optical comparison chip of ToddThorsen, etc. can only detect specific fluorescently labeled bacteria, and cannot realize multi-channel bacterial suspension culture on the chip. Suspension culture is a very important step for microbial screening. It provides specific microbial growth conditions and determines the elimination and retention of certain characteristics of strains.

[0031] Although the microfluidic microbial culture chips disclosed in Chinese Patent Nos. CN201110316751.0 and CN201110142095.7 solve the problem of microorganisms being cultured in suspension on the chip, they can only provide indicators of the growth rate of microorganisms and cannot detect changes in the concentration of specific substances in the culture solution Condition. The detection of the concentration of specific substances in the culture medium is an important basis for judging the quality of the strain, and it is very important for the screening of the strain.

[00...

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Abstract

The invention discloses a microfluidic microbial culture detection chip. The chip comprises a culture layer, an elastic diaphragm layer and a drive layer which are laminated, wherein the elastic diaphragm layer is arranged between the culture layer and the drive layer, more than one culture detection unit is distributed on the culture layer, each culture detection unit comprises an annular culture channel and a detection channel communicated with the culture channel, a circulating drive channel and a detection drive channel are distributed on the drive layer, the circulating drive channel is arranged above the culture channel and is intersected with the culture channel and drives a culture solution in the culture channel to flow circularly, and the detection drive channel comprises at least two drive channels which are arranged above the detection channel and are intersected with the detection channel. The microfluidic microbial culture detection chip can be used for not only realizing suspension culture of microorganism or cells, but also detecting the specified components in the culture solution.

Description

technical field [0001] The invention belongs to the field of microfluidics, and in particular relates to a microfluidic microorganism culture and detection chip. Background technique [0002] Obtaining high-performance strains through microbial screening is an important part of improving the production level of industrial microorganisms. Traditional microbial screening is mainly carried out in shake flasks, including two main steps of microbial suspension culture and culture fluid detection. By inserting each strain into a shake flask for suspension culture, and then carrying out substance detection on the culture solution in the shake flask, the performance of the strain is determined, and excellent strains are selected therefrom. Each batch of screening often requires dozens or even hundreds of shake flasks to be tested. Even so, the number of shake flasks screened is still much lower than the number of strains to be screened, which directly leads to labor-intensive scre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M23/16C12M23/34C12M41/32
Inventor 陈立桅甘明哲
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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