Exendin-4 analog dimer and its preparation method and application
A technology of EXENDIN-4 and analogs, applied in the field of polypeptide complexes, which can solve the problems of short half-life and increased pain for patients
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0068] Embodiment 1: the solid-phase synthesis of polypeptide
[0069] Using the solid-phase peptide synthesis method of the Fmoc strategy (the amino acids used were purchased from Shanghai Jier Company), the CS336X instrument produced by CSBio Company was used to synthesize the EXENDIN-4 analogue monomer of the present invention. The method of synthesis was carried out according to the manufacturer's instruction manual.
[0070] Specifically, the Fmoc solid-phase polypeptide synthesis method is a process in which amino acids are gradually added from the C-terminus, that is, the carboxyl-terminus, to the N-terminus, that is, the amino-terminus, using the solid-phase carrier resin as a carrier.
[0071] In the present invention, the resin is Fmoc-wang resin (or 2-cl-trt resin), and the resin substitution degree is 0.10-0.34.
[0072] The protected amino acids used are: Fmoc-Tyr(tBu)-OH, Fmoc-Ile-OH, Fmoc-leu(tBu)-OH, Fmoc-Ala-OH, Fmoc-Thr(tBu)-OH, Fmoc-Val-OH , Fmoc-Lys(Boc...
Embodiment 2
[0079] Example 2: Hypoglycemic Function of EXENDIN-4 Analog Dimers
[0080] In this embodiment, the Exendin-4 analog monomers used are as follows: SEQ ID NO:2 and SEQ ID NO:2; SEQ ID NO:3 and SEQ ID NO:3; SEQ ID NO:4 and SEQ ID NO:4 ; SEQ ID NO:5 and SEQ ID NO:5; SEQ ID NO:6 and SEQ ID NO:6; SEQ ID NO:7 and SEQ ID7.
[0081] In this embodiment, dimers formed by Exendin-4 analog monomers (containing 1 cysteine) of different sequences can also be used, such as SEQ ID NO: 2 and SEQ ID NO: 3, SEQ ID NO :2 and SEQ ID NO:4, SEQ ID NO:2 and SEQ ID NO:5, SEQ ID NO:2 and SEQ ID NO:6, SEQ ID NO:2 and SEQ ID NO:7, SEQ ID NO:3 and SEQ ID NO:4, SEQ ID NO:3 and SEQ ID NO:5, SEQ ID NO:3 and SEQ ID NO:6, SEQ ID NO:3 and SEQ ID NO:7, SEQ ID NO:4 and SEQ ID NO:5, SEQ ID NO:4 and SEQ ID NO:6, SEQ ID NO:4 and SEQ ID NO:7, SEQ ID NO:5 and SEQ ID NO:6, SEQ ID NO:5 and SEQ ID NO:7 , SEQ ID NO:2 and SEQ ID NO:8.
[0082] Dissolve 1 mg of each of the 9 or 8 dimers in 1 mL of normal saline, and i...
Embodiment 3
[0083] Example 3: Effect of EXENDIN-4 Analog Dimer on Weight Loss in Rats
[0084] In this embodiment, the Exendin-4 analog monomer polypeptides used are as follows: SEQ ID NO:2 and SEQ ID NO:2; SEQ ID NO:3 and SEQ ID NO:3; SEQ ID NO:4 and SEQ IDNO: 4; SEQ ID NO:5 and SEQ ID NO:5; SEQ ID NO:6 and SEQ ID NO:6; SEQ ID NO:7 and SEQ ID7.
[0085] In this embodiment, dimers formed by Exendin-4 analog monomers (containing 1 cysteine) of different sequences can also be used, such as SEQ ID NO: 2 and SEQ ID NO: 3, SEQ ID NO :2 and SEQ ID NO:4, SEQ ID NO:2 and SEQ ID NO:5, SEQ ID NO:2 and SEQ ID NO:6, SEQ ID NO:2 and SEQ ID NO:7, SEQ ID NO:3 and SEQ ID NO:4, SEQ ID NO:3 and SEQ ID NO:5, SEQ ID NO:3 and SEQ ID NO:6, SEQ ID NO:3 and SEQ ID NO:7, SEQ ID NO:4 and SEQ ID NO :5, SEQ ID NO:4 and SEQ ID NO:6, SEQ ID NO:4 and SEQ ID NO:7, SEQ ID NO:5 and SEQ ID NO:6, SEQ ID NO:5 and SEQ ID NO:7, SEQ ID NO:2 and SEQ ID NO:8.
[0086] SD rats (200±20g, purchased from the SLAC Animal Laborat...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com