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Preparation method of efficient organic liquid compound enzyme bacterial fertilizer

A compound enzyme bacteria and organic technology, applied in the direction of fertilization device, fertilizer mixture, application, etc., can solve the problems of soil compaction, achieve the effect of improving soil, absorbing quickly, and promoting growth and development

Inactive Publication Date: 2014-06-04
SHANDONG VOCATIONAL COLLEGE OF SCI & TECH
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AI Technical Summary

Problems solved by technology

[0002] Due to the long-term use of chemical fertilizers, the soil is compacted and a large amount of nutrients are coated, so that the nitrogen, phosphorus and various trace elements in the soil cannot be released and absorbed by plants.

Method used

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  • Preparation method of efficient organic liquid compound enzyme bacterial fertilizer

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] 1. Fermentation of biological agents

[0009] (1) Screening and liquid fermentation culture of phytase-producing strain (Aspergillus niger)

[0010] Preliminary screening of phytase-producing strains: put soybean root soil samples in the surrounding areas of the city into a triangular flask with sterile water, after shaking and standing still, take the supernatant and spread it on the screening medium after dilution. Culture at constant temperature at 37°C for 3-4 days.

[0011] Re-screening of phytase-producing strains: Pick the single colony with the largest transparent circle and put it into the re-screening medium. Cultivate for 2 to 3 days.

[0012] Liquid fermentation culture: the isolated and identified strains are cultivated in liquid enzyme-producing fermentation medium. The inoculum size is 10%, the fermentation temperature is 30°C, and the fermentation time is 84h.

[0013] Among them: the primary screening medium is glucose 30.0g, NH 4 NO 3 5.0g, K...

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Abstract

A preparation method of an efficient organic liquid compound enzyme bacterial fertilizer is characterized in that the formula is prepared by raw materials of urea phosphate (solution), a biological bacterial agent (fermentation liquid), a trace element combination (solution), a glucose plant amino acid liquid, a humic acid liquid, xanthan gum, sodium ethylene diamine tetracetate, an active ingredient and a plant regulator. Through the preparation, a microbiology theory is utilized, acid-producing beneficial bacteria and organic matters are fermented and then are compounded with the active ingredient promoting plant growth and various trace elements required by plants to prepare the product, so as to improve the soil utilization rate, increase the yield, and improve the crop quality. The formula is reasonable, the production cost is low, and the cost can be controlled in 20-50 yuan calculated according to 25 ml per barrel; and the use effect is good, the yield can be increased by 5-10% through applying with 2 barrels per mu, and moreover the quality of agricultural products can be improved. Therefore, the product is worth of popularization.

Description

technical field [0001] The invention relates to the fields of microorganisms and chemistry, in particular to a preparation method of high-efficiency organic liquid compound enzyme fertilizer. Background technique [0002] Due to the long-term use of chemical fertilizers, the soil is compacted and a large amount of nutrients are coated, so that the nitrogen, phosphorus and various trace elements in the soil cannot be released and absorbed by plants. Contents of the invention [0003] The invention proposes a preparation method of high-efficiency organic liquid compound enzyme fertilizer, which is characterized in that: the formula includes raw material urea phosphate (solution), biological bacterial agent (fermentation liquid), trace element sum (solution), glucose factory amino acid Liquid, humic acid liquid, xanthan gum, sodium edetate, active ingredients, plant regulators. [0004] Through the preparation of this invention, using the theory of microbiology, the acid-pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 朱民生郭建东高晓涵
Owner SHANDONG VOCATIONAL COLLEGE OF SCI & TECH
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