Nanopore device for single-molecule sequencing, method of use thereof, and manufacturing method thereof

Abstract

The invention provides a nano-pore apparatus used for single-molecule sequencing and an application method and a manufacturing method thereof. The nano-pore apparatus is used in a single-molecule sequencing device. The single-molecule sequencing device comprises a positive electrolytic bath and a negative electrolytic bath, and the nano-pore apparatus is used for separating the positive electrolytic bath from the negative electrolytic bath. Solid nano-pore structures and a graphene nano-pore structure are combined so as to obtain a sandwich structure; since a solid wine-glass-shaped groove exists in the sandwich structure, the diameter of a graphene nano-pore can be extended, which guarantees that biomolecules have a high capture rate at a low voltage, so the speed of a single molecule passing through the graphene nano-pore can be controlled by lowering down voltage, thereby overcoming the problem of a fast pore passing speed of biomolecules during nano-pore single-molecule sequencing in the prior art and improving temporal resolution during pore passing of the single molecule; the apparatus eventually detects the graphene nano-pore, thereby guaranteeing a good resolution effect of single-molecule detection.

Description

technical field [0001] The invention relates to a nanopore device for single-molecule sequencing, its use method and production method. Background technique [0002] DNA is the blueprint for all life in nature. It contains the genetic information necessary to build other compounds within cells, such as RNA codes and protein formation, and influences the activity of all cells. DNA is a molecular compound of double helix structure composed of two nucleotides based on the principle of complementary pairing. Each nucleotide contains a sugar-phosphate backbone and a base. There are four kinds of bases, namely adenine, thymine, cytosine and guanine. The sequence of these four bases determines the genetic information of all the characteristics of an organism. Detecting the sequence of bases in DNA is playing an increasingly important role in the research of life sciences, human health and medical and health undertakings. As a typical representative of the third-generation sequenci...

AI Technical Summary

Problems solved by technology

At present, the main methods to solve the problem of too fast single-molecule through-hole rate are: on the one hand, use a larger voltage to ensure the single-molecule capture rate; on the other hand, chemically or biologically modify the nanopore, and the modified nanopore will Further control the rate of single molecule movement, but the method of modifying nanopores is difficult to operate, and it is easy to affect the detection effect of single molecules

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