Method of preparing substrate for summer vegetables by utilization of municipal sludge
A technology of municipal sludge and matrix, applied in the biological field, can solve the problems of municipal sludge and achieve the effects of promoting growth, improving absorption and utilization, reducing heating and fermentation time
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preparation example Construction
[0036] The preparation method of microbial fermentation agent described in step 4 comprises the following steps:
[0037] Step 1, preparation of soybean extract
[0038](1) Wash the soybeans, dry them, crush them, and then filter them through a 1mm-diameter sieve to obtain soybean powder for later use;
[0039] (2) Add soybean powder into a phosphate buffer solution with a pH of 5.2 and a molar concentration of 0.05 mol / L, stir evenly to obtain a mixed solution I, and the volume of the phosphate buffer solution corresponding to each gram of soybean powder is 10 mL;
[0040] (3) Put the mixture I prepared in step (2) into a centrifuge with a rotation speed of 6000r / min for 30min, and then dissolve the precipitate in a phosphate buffer solution with a pH of 6.5 and a molar concentration of 0.05mol / L In, then transferred to a centrifuge with a rotating speed of 4000r / min for 15min, the supernatant was collected, and the soybean extract was prepared for subsequent use;
[0041] ...
Embodiment 1
[0045] A kind of method that utilizes city sludge to prepare summer raw vegetable matrix, comprises the following steps:
[0046] Step 1: Add 0.7 mol / L acetic acid and rotten citrus to the urban sludge, stir evenly, and then treat it with an ultrasonic wave with a frequency of 20kHz for 25 minutes, air-dry to obtain the matrix I, and add The quality of acetic acid and orange is respectively 32g, 34g;
[0047] Step 2, put the substrate I into an oven and heat until the temperature of the substrate I is 60°C;
[0048] Step 3: Crush the corn stalks and the heated substrate I, and filter them through a sieve with a pore size of 1-2 mm, mix the filtered corn stalks and the substrate I in a mass ratio of 4:1, and stir evenly to obtain Matrix II, spare;
[0049] Step 4, add water to the matrix II, so that the mass percentage concentration of water in the matrix II is 68-72%, then add the microbial starter to the matrix II, and the ratio of the matrix II to the microbial starter is ...
Embodiment 2
[0063] A kind of method that utilizes city sludge to prepare summer raw vegetable matrix, comprises the following steps:
[0064] Step 1: Add 0.7 mol / L acetic acid and rotten citrus to the urban sludge, stir evenly, and then treat it with an ultrasonic wave with a frequency of 20kHz for 25 minutes, air-dry to obtain the matrix I, and add The quality of acetic acid and orange is respectively 32g, 34g;
[0065] Step 2, put the substrate I into an oven and heat until the temperature of the substrate I is 65°C;
[0066] Step 3: Crush the corn stalks and the heated matrix I, and filter them through a sieve with a pore size of 2 mm, mix the filtered corn straw and the matrix I in a mass ratio of 4:1, and mix well to prepare the matrix II ,spare;
[0067] Step 4, add water to the matrix II, so that the mass percentage concentration of water in the matrix II is 68-72%, then add the microbial starter to the matrix II, and the ratio of the matrix II to the microbial starter is 5:1, an...
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