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A method for decolorizing silkworm chrysalis proteolytically hydrolyzed antioxidant peptides

A technology of proteolysis and anti-oxidation peptides, which is applied in the field of decolorization of silkworm chrysalis proteolysis anti-oxidation peptides, can solve the problems of high cost, secondary pollution, high protein loss rate, etc., and achieve high nitrogen retention rate, good decolorization effect, DPPH inhibition active effect

Active Publication Date: 2016-05-25
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the silkworm chrysalis protein enzymatic hydrolyzate is brown, and if it is directly applied to food additives or medicines, it is not easy to be accepted by consumers in terms of color, so it is necessary to decolorize the enzymatic hydrolyzate
At present, the commonly used decolorization methods include activated carbon method, ion exchange resin decolorization method, organic solvent decolorization method, etc., but these methods lead to high protein loss rate, high cost or secondary pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of silkworm chrysalis protein enzymatic hydrolysis blood pressure-lowering peptide liquid: dissolve 40g silkworm chrysalis protein powder into 2L water to form silkworm chrysalis protein water-soluble, add neutral protease as hydrolytic enzyme, the addition amount of neutral protease is 1% of silkworm chrysalis protein quality, controlled at Enzymatically hydrolyze for 4 hours under the environment of pH 7.0, keep the pH value constant with NaOH solution, and inactivate the enzyme to obtain silkworm chrysalis proteolytic blood pressure lowering peptide liquid. It was determined that the DPPH inhibitory activity was 52.50%, and the protein content of the enzymatic hydrolyzate was 10mg·L -1 , The detected amount of DPPH inhibitory active protein was 200 μg.

[0022] 2. Decolorization of silkworm chrysalis protease hydrolyzed hypotensive peptide solution

[0023] Adjust the pH value of the prepared silkworm chrysalis proteolysis blood pressure lowering pept...

Embodiment 2

[0025] 1. The preparation of the antihypertensive peptide liquid hydrolyzed by silkworm chrysalis proteolysis is the same as that in Example 1.

[0026] 2. Decolorization of silkworm chrysalis protease hydrolyzed hypotensive peptide solution

[0027] The pH value of the silkworm chrysalis proteolytic blood pressure lowering peptide solution prepared in Example 1 was adjusted to 6.5, and the mass ratio of bentonite, activated clay and alkaline calcium-based bentonite in the decolorizing agent was 1:0.5:1. The decolorizing agent and silkworm chrysalis protease The mass-to-volume ratio of the antihypertensive peptide solution is 20g / L. During ultrasonic oscillation, adjust the ultrasonic initial temperature to 35°C, ultrasonic power to 35W, and decolorize for 20 minutes; centrifuge at a speed of 4000r·min -1 After centrifugation for 20 minutes, the obtained supernatant was decolorized silkworm chrysalis proteolytic blood pressure-lowering peptide solution. After testing, the fina...

Embodiment 3

[0029] 1. The preparation of the antihypertensive peptide liquid hydrolyzed by silkworm chrysalis proteolysis is the same as that in Example 1.

[0030] 2. Decolorization of silkworm chrysalis protease hydrolyzed hypotensive peptide solution

[0031] The pH value of the silkworm chrysalis proteolytic blood pressure-lowering peptide solution prepared in Example 1 was adjusted to 7.0, and the mass ratio of bentonite, activated clay and alkaline calcium-based bentonite in the decolorizing agent was 1:1:1. The decolorizing agent and silkworm chrysalis protease The mass-to-volume ratio of the antihypertensive peptide solution is 25g / L. During ultrasonic oscillation, adjust the ultrasonic initial temperature to 40°C, ultrasonic power 30W, and decolorize for 30 minutes; centrifugal separation speed 4000r·min -1 After centrifugation for 20 minutes, the obtained supernatant was decolorized silkworm chrysalis proteolytic blood pressure-lowering peptide solution. After testing, the final...

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Abstract

The invention discloses a decolorizing method of a silkworm chrysalis proteolysis antioxidative peptide. The decolorizing method comprises the following steps: adjusting pH value of silkworm chrysalis proteolysis antioxidative peptide liquid to 5-8, adding a bentonite, activated clay and alkaline Ca-bentonite mixture as a decolorizing agent, wherein the mass volume ratio of the decolorizing agent to the silkworm chrysalis proteolysis antioxidative peptide liquid is 5g / L-30g / L, carrying out ultrasonic oscillation decolorizing at the temperature of 35-45 DEG C for 20-30 minutes, and carrying out centrifugal separation so as to obtain liquid supernatant which is the decolorized silkworm chrysalis proteolysis antioxidative peptide. The decolorizing method has the advantages that the bentonite, activated clay and alkaline Ca-bentonite mixture is used as the decolorizing agent, so that the antioxidant inhibitory activity (1,1-diphenyl-2-picryl-hydrazyl inhibitory activity) is also effectively retained while the relatively high decolorization rate is ensured; and the decolorizing method is simple, practicable in operation and mild in treatment process and can be widely applied to the production process of silkworm chrysalis polypeptides and other bioactive peptides.

Description

technical field [0001] The invention relates to the technical field of food decolorization, in particular to a decolorization method of silkworm chrysalis proteolytically hydrolyzed antioxidant peptides. Background technique [0002] Silkworm chrysalis (Silkwormpupa) is the chrysalis of the Bombyx moth of the silk moth family insect, and is a large by-product of the silk industry. According to the statistics of the Ministry of Commerce of the People's Republic of China, in 2009 the output of dried silkworm cocoons, the raw material of silk, reached 782,100 tons. Half of the dry weight of silkworm cocoons is silkworm chrysalis. Due to the lack of deep processing technology, a large number of silkworm chrysalis are used as feed and fertilizer, and the resource utilization rate and added value are low. The silkworm chrysalis protein accounts for 45%-50% of the dry silkworm chrysalis mass. It has been determined that the silkworm chrysalis protein contains 18 kinds of amino ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C12P21/06
Inventor 赵钟兴刘杰婷谢美萱韦藤幼廖丹葵孙建华童张法
Owner GUANGXI UNIV
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